May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

Name: Caspase-8 Antibody (84131)
Brand: Novus Biologicals
SKU: MAB704
Rating: 4 (1 reviews)

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Accepted Answer

In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB
Clone	84131
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Formulation	Size	Price
Carrier Free MAB704-SP

Immunogen	E. coli-derived recombinant human Caspase-8 aa 234-391
Specificity	Detects human Caspase-8 precursor in Western blots and a 42 kDa doublet generated during apoptosis.
Source	N/A
Isotype	IgG2a
Clonality	Monoclonal
Host	Mouse
Gene	CASP8
Purity Statement	Protein A or G purified from ascites
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

Caspase-8 Antibody (84131)

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Publications using MAB704	Applications	Species
J Hou, R Zhao, W Xia, CW Chang, Y You, JM Hsu, L Nie, Y Chen, YC Wang, C Liu, WJ Wang, Y Wu, B Ke, JL Hsu, K Huang, Z Ye, Y Yang, X Xia, Y Li, CW Li, B Shao, JA Tainer, MC Hung PD-L1-mediated gasdermin C expression switches apoptosis to pyroptosis in cancer cells and facilitates tumour necrosis Nat. Cell Biol., 2020-09-14;0(0):. 2020-09-14 [PMID: 32929201] (Western Blot, Human)	Western Blot	Human
L Xu, X Lin, Y Zheng, H Zhou Silencing of heat shock protein 27 increases the radiosensitivity of non?small cell lung carcinoma cells Mol Med Rep, 2019-05-22;0(0):. 2019-05-22 [PMID: 31115576] (Western Blot, Human)	Western Blot	Human
Mongiat M, Ligresti G, Marastoni S, Lorenzon E, Doliana R, Colombatti A Regulation of the extrinsic apoptotic pathway by the extracellular matrix glycoprotein EMILIN2. Mol. Cell. Biol., 2007-08-13;27(20):7176-87. 2007-08-13 [PMID: 17698584] (Western Blot, Human)	Western Blot	Human
Uchida M, Iwase M, Takaoka S, Yoshiba S, Kondo G, Watanabe H, Ohashi M, Nagumo M, Shintani S Enhanced susceptibility to tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis in oral squamous cell carcinoma cells treated with phosphatidylinositol 3-kinase inhibitors. Int. J. Oncol., 2007-05-01;30(5):1163-71. 2007-05-01 [PMID: 17390018] (Western Blot, Human)	Western Blot	Human
Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaan-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells. J. Cell. Sci., 2006-09-26;119(0):4247-56. 2006-09-26 [PMID: 17003109] (ICC, Human)	ICC	Human

	Verified Customer 02/25/2015
Application:	WB
Species:	Human