Does this antibody work for immunofluorescence staining in mouse tissue?

Name: Dicer Antibody
Brand: Novus Biologicals
SKU: NB200-591
Price: 329 USD

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Accepted Answer

This antibody lists both Mouse and IF. We guarantee all of our products to work in all species and applications listed.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Immunogen	Rabbit anti-mouse Dicer polyclonal antibody was raised against a peptide corresponding to amino acids 132-150 and 1017-1033 of mouse Dicer (EDL18787).
Localization	IMG-538
Specificity	In mouse ovary, a 217 kDa band is observed.
Predicted Species	Rat (90%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	DICER1
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1.0 mg/ml
Purity	Protein G purified

Publications using NB200-591	Applications	Species
Grelier G, N Voirin, A Ay et al. Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype. B J Cancer 101:673-683 (2009).
Lei D, Shao Z, Zhou X, Yuan H. Synergistic neuroprotective effect of rasagiline and idebenone against retinal ischemia-reperfusion injury via the Lin28-let-7-Dicer pathway. Oncotarget. 2018-02-23 [PMID: 29552298] (WB, Mouse)	WB	Mouse
Simeoli R, Montague K, Jones HR et al. Exosomal cargo including microRNA regulates sensory neuron to macrophage communication after nerve trauma Nat Commun. 2017-11-24 [PMID: 29176651] (WB, Mouse)	WB	Mouse
de Almeida DC, Bassi EJ, Azevedo H et al. A Regulatory miRNA-mRNA Network Is Associated with Tissue Repair Induced by Mesenchymal Stromal Cells in Acute Kidney Injury Front Immunol. 2016-01-18 [PMID: 28096802] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Lu XF, Zhou YJ, Zhang L et al. Loss of Dicer1 impairs hepatocyte survival and leads to chronic inflammation and progenitor cell activation World J. Gastroenterol. 2015-06-07 [PMID: 26074697] (IHC-P, Mouse)	IHC-P	Mouse
Bhattachariya, A, Dahan, D Et al. Expression of microRNAs is essential for arterial myogenic tone and pressure-induced activation of the PI3-kinase/Akt pathway. Cardiovasc Res 2014-02-01 [PMID: 24233972] (IF/IHC, Rat)	IF/IHC	Rat
Chiappinelli KB, Haynes BC, Brent MR et al. Reduced DICER1 elicits an interferon response in endometrial cancer cells. Mol Cancer Res. 2012-03-01 [PMID: 22252463] (WB, Human)	WB	Human
Grelier G, Voirin N, Ay AS et al. Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype. Br J Cancer. 2009-08-18 [PMID: 19672267] (WB, IHC-P) Details: 1. WB: Four human breast cancer cell lines; HMEC, HMEC+hTERT, HMEC+LT+hTERT, and HMLER, Fig 2A. 2. WB: Five mouse mammary cell lines; 67NR, 168FARN, 4TO7, 66c14, and 4T1, Fig 3A. 3. IHC (Paraffin): Normal breast and ductal carcinoma tissue, Fig 4A, 4B, &	WB, IHC-P
Chen JF, Murchison EP, Tang R et al. Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure. Proc Natl Acad Sci U S A. 2008-02-12 [PMID: 18256189]
Poleshko A, Palagin I, Zhang R et al. Identification of cellular proteins that maintain retroviral epigenetic silencing: evidence for an antiviral response. J Virol. 2008-03-01 [PMID: 18094192] (WB) Details: WB: HeLa TI-C cells treated with Dicer siRNA, Fig 3C. Note: The (IMG-538) Dicer pAb was siRNA validated by WB in HeLa TI-C cells, Fig 3C.	WB
Barbato C, Ciotti MT, Serafino A et al. Dicer expression and localization in post-mitotic neurons. Brain Res. 2007-10-17 [PMID: 17888888] (WB, Rat) Details: Rat neuronal primary cultures from 8 day old Wistar rats. IF (Figs 1, 2B, 2C, 3A, 4-8); WB (Figs 2A, 7A). Notes: 1. Dicer was detected as a single band just below the 220 kDa marker in total cell lysates from hippocampal, cortical, and cerebellar granule	WB	Rat
Grivna ST, Pyhtila B, Lin H. MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis. Proc Natl Acad Sci U S A. 2006-09-05 [PMID: 16938833]
Lugli G, Larson J, Martone ME et al. Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner. J Neurochem. 2005-08-01 [PMID: 16092937] (WB, Human, Mouse) Details: WB: Recombinant human dicer and a cytosolic fraction of adult mouse brain (data not shown). 1. Recombinant dicer was recognized at ~220 and 125 kDa bands. The 220 kDa band represents full length dicer and the 125 kDa a fragmented form of dicer. 2. Mouse b	WB	Human, Mouse
Doi N et al. Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer eIF2C translation initiation factors. Curr Biol 13:41-6. 2003-01-01 [PMID: 12526743]
Provost P et al. Dicer is required for chromosome segregation gene silencing in fission yeast cells. Proc Natl Acad Sci U S A 99:16648-53. 2002-01-01 [PMID: 12482946]
Zhang H et al. Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP. EMBO J 21:5875-85. 2002-01-01 [PMID: 12411505]
Show All 16 Publications. Collapse Publications.

Gene Symbol	DICER1
Entrez	192119(Mouse)
Uniprot	Q8R418(Mouse)

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