![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0003.jpg "Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 2A8H3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human ZEB1 (AA: 967-1108) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ZEB1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 124 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for ZEB1 Antibody (NBP2-37329)Find related products by research area.
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Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1 By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can... Read full blog post. |
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Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
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Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel... Read full blog post. |
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CD63: is it pro-metastatic or anti-metastatic? CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,... Read full blog post. |
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Beta Catenin in Cell Adhesion and T-cell Signaling Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi... Read full blog post. |
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| Gene Symbol | ZEB1 |
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunofluorescence analysis of Hela cells using ZEB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunocytochemistry-Immunofluorescence-NBP2-37329-img0004.jpg "Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunofluorescence analysis of Hela cells using ZEB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0007.jpg "Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZEB1 mouse mAb with DAB staining.")
![Flow Cytometry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Flow-Cytometry-NBP2-37329-img0005.jpg "Flow Cytometry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).")
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against human ZEB1 recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0002.jpg "Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against human ZEB1 recombinant protein. (Expected MW is 41.7 kDa)")
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0006.jpg "Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining.")
![ELISA: ZEB1 Antibody (2A8H3) [NBP2-37329] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-ELISA-NBP2-37329-img0001.jpg "ELISA: ZEB1 Antibody (2A8H3) [NBP2-37329] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")