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GEMIN2 Antibody - BSA Free

Images

 
Western Blot: GEMIN2 Antibody [NBP1-77177] - Analysis in HeLa cell lysate with antibody at (A) 0.5 and (B) 1ug/mL.
Immunocytochemistry/ Immunofluorescence: GEMIN2 Antibody [NBP1-77177] - HeLa cells at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: GEMIN2 Antibody [NBP1-77177] - HeLa cells at 4 ug/mL.
Immunocytochemistry/ Immunofluorescence: GEMIN2 Antibody - BSA Free [NBP1-77177] - Immunofluorescence of GEMIN2 in HeLa cells with GEMIN2 antibody at 20 u/mL.
Immunocytochemistry/ Immunofluorescence: GEMIN2 Antibody - BSA Free [NBP1-77177] - Immunocytochemistry of GEMIN2 in HeLa cells with GEMIN2 antibody at 4 u/mL.
Immunocytochemistry/ Immunofluorescence: GEMIN2 Antibody - BSA Free [NBP1-77177] - Immunocytochemistry of GEMIN2 in HeLa cells with GEMIN2 antibody at 4 u/mL.
Immunocytochemistry/ Immunofluorescence: GEMIN2 Antibody - BSA Free [NBP1-77177] - Immunofluorescence of GEMIN2 in HeLa cells with GEMIN2 antibody at 20 u/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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GEMIN2 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human SIP1. The immunogen is located within the last 50 amino acids of SIP1. Amino Acid Squence: VSRYFDQRDLADEPS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GEMIN2
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 4-20 ug/ml
  • Western Blot 0.5-1 ug/ml
Theoretical MW
31.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
GEMIN2 Peptide (NBP1-77177PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for GEMIN2 Antibody - BSA Free

  • Component of gems 2
  • Gemin-2
  • GEMIN2survival of motor neuron protein-interacting protein 1
  • SIP1-delta
  • SMN interacting protein 1-delta
  • SMN-interacting protein 1
  • survival of motor neuron protein interacting protein 1

Background

SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GEMIN2 Antibody (NBP1-77177) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GEMIN2
Entrez
Uniprot