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Wnt3 Antibody (OTI1G7) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1G7
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74869AF350
Alexa Fluor 405
NBP2-74869AF405
Alexa Fluor 488
NBP2-74869AF488
Alexa Fluor 532
NBP2-74869AF532
Alexa Fluor 594
NBP2-74869AF594
Alexa Fluor 647
NBP2-74869AF647
Alexa Fluor 700
NBP2-74869AF700
Alexa Fluor 750
NBP2-74869AF750
Biotin
NBP2-74869B
DyLight 350
NBP2-74869UV
DyLight 405
NBP2-74869V
DyLight 488
NBP2-74869G
DyLight 550
NBP2-74869R
DyLight 594
NBP2-74869DL594
DyLight 650
NBP2-74869C
DyLight 680
NBP2-74869FR
DyLight 755
NBP2-74869IR
FITC
NBP2-74869F
HRP
NBP2-74869H
Janelia Fluor 525
NBP2-74869JF525
Janelia Fluor 549
NBP2-74869JF549
Janelia Fluor 585
NBP2-74869JF585
Janelia Fluor 635
NBP2-74869JF635
Janelia Fluor 646
NBP2-74869JF646
Janelia Fluor 669
NBP2-74869JF669
Unconjugated
NBP2-46367
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46367
Azide and BSA Free
NBP2-74869
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Wnt3 Antibody (OTI1G7) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Human recombinant protein fragment corresponding to amino acids 128-355 of human WNT3 (NP_110380) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
WNT3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Wnt3 Antibody (OTI1G7) [CoraFluor™ 1]

  • INT4
  • INT4Proto-oncogene Int-4 homolog
  • MGC131950
  • MGC138321
  • MGC138323
  • proto-oncogene Wnt-3
  • TETAMS
  • wingless-type MMTV integration site family, member 3
  • WNT-3 proto-oncogene protein
  • WNT3
  • Wnt-3

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol WNT3