Recombinant Mouse Wnt-9b Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1-3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5-7). Induction is dependent on beta -catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Binding Assay, Bioassay, Cell Culture.

Showing Publications 1 - 9 of 9.

Publications using 3669-WN/CF	Applications	Species
S Gupta, R Kawaguchi, E Heinrichs, S Gallardo, S Castellano, I Mandric, BG Novitch, SJ Butler In�vitro atlas of dorsal spinal interneurons reveals Wnt signaling as a critical regulator of progenitor expansion Oncogene, 2022-07-19;40(3):111119. 2022-07-19 [PMID: 35858555] (Bioassay, Human)	Bioassay	Human
CY Chang, MZ Liang, CC Wu, PY Huang, HI Chen, SF Yet, JW Tsai, CF Kao, L Chen WNT3A Promotes Neuronal Regeneration upon Traumatic Brain Injury Int J Mol Sci, 2020-02-21;21(4):. 2020-02-21 [PMID: 32098078] (Cell Culture, Rat)	Cell Culture	Rat
KK Dickinson, LC Hammond, CM Karner, ND Hastie, TJ Carroll, P Goodyer Molecular determinants of WNT9b responsiveness in nephron progenitor cells PLoS ONE, 2019-04-12;14(4):e0215139. 2019-04-12 [PMID: 30978219] (Bioassay, Mouse)	Bioassay	Mouse
Kilander M, Dahlstrom J, Schulte G Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 2014-05-27;26(9):1943-9. 2014-05-27 [PMID: 24873871] (Bioassay, Human)	Bioassay	Human
Okamoto M, Udagawa N, Uehara S, Maeda K, Yamashita T, Nakamichi Y, Kato H, Saito N, Minami Y, Takahashi N, Kobayashi Y Noncanonical Wnt5a enhances Wnt/beta-catenin signaling during osteoblastogenesis. Sci Rep, 2014-03-27;4(0):4493. 2014-03-27 [PMID: 24670389] (Bioassay, Mouse)	Bioassay	Mouse
Thrasivoulou C, Millar M, Ahmed A Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways. J Biol Chem, 2013-10-24;288(50):35651-9. 2013-10-24 [PMID: 24158438] (Bioassay, Human)	Bioassay	Human
Goel S, Chin EN, Fakhraldeen SA Both LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in mammary epithelial cells and fibroblasts. J. Biol. Chem., 2012-03-20;287(20):16454-66. 2012-03-20 [PMID: 22433869] (Bioassay, Mouse)	Bioassay	Mouse
Gong Y, Bourhis E, Chiu C, Stawicki S, DeAlmeida VI, Liu BY, Phamluong K, Cao TC, Carano RA, Ernst JA, Solloway M, Rubinfeld B, Hannoush RN, Wu Y, Polakis P, Costa M Wnt isoform-specific interactions with coreceptor specify inhibition or potentiation of signaling by LRP6 antibodies. PLoS ONE, 2010-09-13;5(9):e12682. 2010-09-13 [PMID: 20856934] (Binding Assay, Human)	Binding Assay	Human
Bourhis E, Tam C, Franke Y, Bazan JF, Ernst J, Hwang J, Costa M, Cochran AG, Hannoush RN Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J. Biol. Chem., 2010-01-21;285(12):9172-9. 2010-01-21 [PMID: 20093360] (Binding Assay, Human)	Binding Assay	Human

Array 3669-WN/CF

• Wnt-9b Antibodies
• Wnt-9b ELISA Kits
• Wnt-9b Lysate
• Wnt-9b Protein
• Wnt-9b Proteins and Enzymes