Home » Wnt3 » Wnt3 Antibodies » Wnt3 Antibody (OTI1G7) - Azide and BSA Free
Novus Biologicals products are now on bio-techne.com

Wnt3 Antibody (OTI1G7) - Azide and BSA Free

 Submit a Review

Images

 
(
Enlarge)
Western Blot: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3.
Western Blot: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WNT3.
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Wnt3 Antibody (OTI1G7) - Azide and BSA Free [NBP2-74869] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI1G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74869AF350
Alexa Fluor 405
NBP2-74869AF405
Alexa Fluor 488
NBP2-74869AF488
Alexa Fluor 532
NBP2-74869AF532
Alexa Fluor 594
NBP2-74869AF594
Alexa Fluor 647
NBP2-74869AF647
Alexa Fluor 700
NBP2-74869AF700
Alexa Fluor 750
NBP2-74869AF750
Biotin
NBP2-74869B
CoraFluor 1
NBP2-74869CL1
DyLight 350
NBP2-74869UV
DyLight 405
NBP2-74869V
DyLight 488
NBP2-74869G
DyLight 550
NBP2-74869R
DyLight 594
NBP2-74869DL594
DyLight 650
NBP2-74869C
DyLight 680
NBP2-74869FR
DyLight 755
NBP2-74869IR
FITC
NBP2-74869F
HRP
NBP2-74869H
Janelia Fluor 525
NBP2-74869JF525
Janelia Fluor 549
NBP2-74869JF549
Janelia Fluor 585
NBP2-74869JF585
Janelia Fluor 635
NBP2-74869JF635
Janelia Fluor 646
NBP2-74869JF646
Janelia Fluor 669
NBP2-74869JF669
Unconjugated
NBP2-46367
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46367
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Wnt3 Antibody (OTI1G7) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 128-355 of human WNT3 (NP_110380) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
WNT3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
37.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Wnt3 Antibody (OTI1G7) - Azide and BSA Free

  • INT4
  • INT4Proto-oncogene Int-4 homolog
  • MGC131950
  • MGC138321
  • MGC138323
  • proto-oncogene Wnt-3
  • TETAMS
  • wingless-type MMTV integration site family, member 3
  • WNT-3 proto-oncogene protein
  • WNT3
  • Wnt-3

Background

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Wnt-3a
[Unconjugated]
5036-WN
Bioactivity Wnt-3a [Unconjugated]
Bioactivity Wnt-3a [Unconjugated]
Cell Culture Wnt-3a [Unconjugated]
(363 Publications)
Species: Hu
Applications: BA, BA
beta-Catenin
Antibody [Unconjugate
AF1329
Western Blot beta-Catenin Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]
Simple Western beta-Catenin Antibody [Unconjugated]
(2 Reviews)
(33 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
Wnt-3a
[Unconjugated]
5036-WN
Bioactivity Wnt-3a [Unconjugated]
Bioactivity Wnt-3a [Unconjugated]
Cell Culture Wnt-3a [Unconjugated]
(363 Publications)
Species: Hu
Applications: BA, BA
NRAMP1/SLC11A1
Antibody (910825)
MAB8400
Western Blot NRAMP1/SLC11A1 Antibody (910825)
Flow Cytometry NRAMP1/SLC11A1 Antibody (910825)
Species: Hu
Applications: CyTOF-ready, Flow, WB
Dkk-1
[Unconjugated]
5439-DK
Cell Culture Dkk-1 [Unconjugated]
Cell Culture Dkk-1 [Unconjugated]
Bioactivity Dkk-1 [Unconjugated]
(90 Publications)
Species: Hu
Applications: BA
Wnt-5a
[Unconjugated]
645-WN
Bioactivity Wnt-5a [Unconjugated]
(180 Publications)
Species: Hu, Mu
Applications: BA
Wnt-1 Antibody
(10C8) - BSA Free
NBP1-51575
Immunocytochemistry/ Immunofluorescence Wnt-1 Antibody (10C8) - BSA Free
Immunohistochemistry-Paraffin Wnt-1 Antibody (10C8) - BSA Free
Western Blot Wnt-1 Antibody (10C8) - BSA Free
(2 Publications)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
LEF1 Antibody (3H5)
H00051176-M01
Western Blot LEF1 Antibody (3H5)
Sandwich ELISA LEF1 Antibody (3H5)
Western Blot LEF1 Antibody (3H5)
(3 Publications)
Species: Hu, Mu
Applications: ELISA, ICC/IF, KD, PLA, S-ELISA, WB
HNF-4 alpha/NR2A1
Antibody
NBP1-89679
Immunohistochemistry-Paraffin HNF-4 alpha/NR2A1 Antibody
Immunohistochemistry-Paraffin HNF-4 alpha/NR2A1 Antibody
Western Blot HNF-4 alpha/NR2A1 Antibody
(1 Review)
(4 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
BMP-4
[Unconjugated]
314-BP
SDS-PAGE BMP-4 [Unconjugated]
Bioactivity BMP-4 [Unconjugated]
(516 Publications)
Species: Hu
Applications: BA, BA
Wnt-9b
3669-WN/CF
Wnt-9b
(9 Publications)
Species: Mu
Applications: BA
BMP-2
[Unconjugated]
355-BM
Bioactivity BMP-2 [Unconjugated]
(190 Publications)
Species: Hu, Mu, Rt
Applications: BA
LRP-5 Antibody
AF6646
Western Blot LRP-5 Antibody
Species: Hu
Applications: WB
LRP-6 Antibody
(255302) [Unconjugate
MAB1505
Flow Cytometry LRP-6 Antibody (255302) [Unconjugated]
Immunocytochemistry LRP-6 Antibody (255302) [Unconjugated]
(6 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC
Frizzled-1 Antibody
AF1120
Immunohistochemistry Frizzled-1 Antibody
(5 Publications)
Species: Mu
Applications: IHC, WB
beta-Catenin
Antibody [Unconjugate
AF1329
Western Blot beta-Catenin Antibody [Unconjugated]
Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]
Simple Western beta-Catenin Antibody [Unconjugated]
(2 Reviews)
(33 Publications)
Species: Hu, Mu, Rt
Applications: ChIP, CyTOF-ready, ICC, IHC, ICFlow, Simple Western, WB
Axin-1 Antibody
AF3287
Western Blot Axin-1 Antibody
(15 Publications)
Species: Hu, Mu, Rt
Applications: WB
FGF-8
[Unconjugated]
423-F8
SDS-PAGE FGF-8 [Unconjugated]
Bioactivity FGF-8 [Unconjugated]
Cell Culture FGF-8 [Unconjugated]
(1 Review)
(62 Publications)
Species: Hu, Mu
Applications: BA

Publications for Wnt3 Antibody (NBP2-74869) (0)

There are no publications for Wnt3 Antibody (NBP2-74869).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Wnt3 Antibody (NBP2-74869) (0)

There are no reviews for Wnt3 Antibody (NBP2-74869). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Wnt3 Antibody (NBP2-74869) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Wnt3 Products

Research Areas for Wnt3 Antibody (NBP2-74869)

Find related products by research area.

Blogs on Wnt3

There are no specific blogs for Wnt3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Wnt3 Antibody (OTI1G7) - Azide and BSA Free and receive a gift card or discount.


Bioinformatics

Gene Symbol WNT3