Novus Biologicals products are now on bio-techne.com

WHSC2 Antibody (6B11H8) - BSA Free

Images

 
Western Blot: WHSC2 Antibody (6B11H8) [NBP2-37394] - Western blot analysis using WHSC2 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A549 (5), SPC-A-1 (6) cell lysate, and Rat brain (4) tissue lysate.
Immunocytochemistry/ Immunofluorescence: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunofluorescence analysis of HeLa cells using WHSC2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WHSC2 mouse mAb with DAB staining.
Flow Cytometry: WHSC2 Antibody (6B11H8) [NBP2-37394] - Flow cytometric analysis of HEK293 cells using WHSC2 mouse mAb (green) and negative control (purple).
Western Blot: WHSC2 Antibody (6B11H8) [NBP2-37394] - Western blot analysis using WHSC2 mAb against human WHSC2 recombinant protein. (Expected MW is 50.2 kDa)
Western Blot: WHSC2 Antibody (6B11H8) [NBP2-37394] - Western blot analysis using WHSC2 mAb against HEK293 (1) and WHSC2 (AA: 280-511)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: WHSC2 Antibody (6B11H8) [NBP2-37394] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using WHSC2 mouse mAb with DAB staining.
ELISA: WHSC2 Antibody (6B11H8) [NBP2-37394] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
6B11H8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

WHSC2 Antibody (6B11H8) - BSA Free Summary

Immunogen
Purified recombinant fragment of human WHSC2 (AA: 280-511) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
WHSC2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for WHSC2 Antibody (6B11H8) - BSA Free

  • FLJ10442
  • negative elongation factor A
  • NELFA
  • NELF-AFLJ25112
  • Wolf-Hirschhorn syndrome candidate 2 protein
  • Wolf-Hirschhorn syndrome candidate 2

Background

WHSC2 is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-52963
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-81759
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
H00051497-B01P
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-83508
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB500-171
Species: Hu, I, Mu, Rt, Xp, Ye
Applications: ChIP, IB, ICC/IF, IHC, IHC-P, Single-Cell Western, WB
H00003954-M03
Species: Hu, Mu
Applications: ELISA, Func, ICC/IF, IHC, IHC-P, IP, WB
NBP1-88036
Species: Hu
Applications: IHC, IHC-P, WB
AF1759
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
NBP1-82924
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
H00007884-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, WB
NBP1-32622
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
MAB4614
Species: Hu
Applications: CyTOF-ready, Flow, KO
H00006827-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP2-37357
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
NB100-402
Species: Ca, Hu, Mu, Po, Rt, Xp, Ze
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
AF972
Species: Hu
Applications: ELISA, IHC, KO, Simple Western, WB
NB100-91273
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-13785
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC-P, IP, PA, WB
NBP2-67895
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
NBP2-37394
Species: Hu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready

Publications for WHSC2 Antibody (NBP2-37394) (0)

There are no publications for WHSC2 Antibody (NBP2-37394).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for WHSC2 Antibody (NBP2-37394) (0)

There are no reviews for WHSC2 Antibody (NBP2-37394). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for WHSC2 Antibody (NBP2-37394) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional WHSC2 Products

Research Areas for WHSC2 Antibody (NBP2-37394)

Find related products by research area.

Blogs on WHSC2

There are no specific blogs for WHSC2, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our WHSC2 Antibody (6B11H8) - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol WHSC2