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LETM1 Antibody (6F7)

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Western Blot: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of LETM1 expression in transfected 293T cell line by LETM1 monoclonal antibody (M03), clone 6F7. Lane 1: LETM1 transfected lysatE (83.4 KDa). Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of monoclonal antibody to LETM1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of monoclonal antibody to LETM1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml
Western Blot: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of LETM1 expression in HeLa (Cat # L013V1).
ELISA: LETM1 Antibody (6F7) [H00003954-M03] - Detection limit for recombinant GST tagged LETM1 is approximately 0.03ng/ml as a capture antibody.
Immunoprecipitation: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of LETM1 transfected lysate using anti-LETM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LETM1 rabbit polyclonal antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Func, ICC/IF, IHC, IP
Clone
6F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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LETM1 Antibody (6F7) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
LETM1 (NP_036450, 601 a.a. ~ 708 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ESKASKRLTKRVQQMIGQIDGLISQLEMDQQAGKLAPANGMPTGENVISVAELINAMKQVKHIPESKLTSLAAALDENKDGKVNIDDLVKVIELVDKEDVHISTSQVA
Specificity
LETM1 - leucine zipper-EF-hand containing transmembrane protein 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LETM1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Publications
Read Publications using H00003954-M03.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LETM1 Antibody (6F7)

  • LETM1 and EF-hand domain-containing protein 1, mitochondrial
  • leucine zipper-EF-hand containing transmembrane protein 1
  • Leucine zipper-EF-hand-containing transmembrane protein 1
  • Mdm38 homolog

Background

The LETM1 gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LETM1 Antibody (H00003954-M03)(12)


Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using H00003954-M03 Applications Species
Quangdon T, Hyunji L, Jae J et al. Emerging role of LETM1/GRP78 axis in lung cancer. Cell Death Dis. 2022-06-10 [PMID: 35680871]
Eri M, Yusuke K, Yuzuki K et al. Mitochondrial LETM1 drives ionic and molecular clock rhythms in circadian pacemaker neurons. Cell Rep. 2022-05-10 [PMID: 35545046]
Nakamura S, Matsui A, Akabane S et al. The mitochondrial inner membrane protein LETM1 modulates cristae organization through its LETM domain. Commun Biol. 2020-03-05 [PMID: 32139798]
Piao L, Feng Y, Yang Z et al. LETM1 is a potential cancer stem-like cell marker and predicts poor prognosis in colorectal adenocarcinoma. Pathol Res Pract. 2019-05-05 [PMID: 31101574]
Yang Z, Ni W, Cui C et al. Identification of LETM1 as a marker of cancer stem-like cells and predictor of poor prognosis in esophageal squamous cell carcinoma. Hum Pathol 2018-07-18 [PMID: 30031102]
Li N, Zheng Y, Xuan C et al. LETM1 overexpression is correlated with the clinical features and survival outcome of breast cancer. Int J Clin Exp Pathol 2015-10-15 [PMID: 26722481]
Doonan PJ, Chandramoorthy HC, Hoffman NE et al. LETM1-dependent mitochondrial Ca2+ flux modulates cellular bioenergetics and proliferation. FASEB J. 2014-07-30 [PMID: 25077561]
Chen L, Yang Y, Liu S et al. High Expression of Leucine Zipper-EF-Hand Containing Transmembrane Protein 1 Predicts Poor Prognosis in Head and Neck Squamous Cell Carcinoma. BioMed Research International. 2014-01-01 [PMID: 24689060]
Shin JY, Chung YS, Kang B et al. Co-delivery of LETM1 and CTMP synergistically inhibits tumor growth in H-ras12V liver cancer model mice. Cancer Gene Ther. 2013-02-08 [PMID: 23392203]
Piao L, Li Y, Kim SJ et al. Association of LETM1 MRPL36 Contributes to the Regulation of Mitochondrial ATP Production Necrotic Cell Death. Cancer Res 69(8):3397-404. 2009-04-15 [PMID: 19318571]
Show All 12 Publications.

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Bioinformatics

Gene Symbol LETM1
Entrez
OMIM
Uniprot