LETM1 Antibodies
We offer LETM1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Simple Western, Western Blot. Each LETM1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our LETM1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the LETM1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our LETM1 polyclonal antibodies and browse our LETM1 monoclonal antibody catalog.
Alternate Names for LETM1 Antibodies
anti-LETM1 antibody, anti-LETM1 and EF-hand domain-containing protein 1, mitochondrial antibody, anti-leucine zipper-EF-hand containing transmembrane protein 1 antibody, anti-Leucine zipper-EF-hand-containing transmembrane protein 1 antibody, anti-Mdm38 homolog antibody
8 Results for "letm1" in Primary Antibodies
![Western Blot: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of LETM1 expression in transfected 293T cell line by LETM1 monoclonal antibody (M03), clone 6F7. Lane 1: LETM1 transfected lysatE (83.4 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/LETM1-Antibody-6F7-Western-Blot-H00003954-M03-img0009.jpg "Western Blot: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of LETM1 expression in transfected 293T cell line by LETM1 monoclonal antibody (M03), clone 6F7. Lane 1: LETM1 transfected lysatE (83.4 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of monoclonal antibody to LETM1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/LETM1-Antibody-6F7-Immunocytochemistry-Immunofluorescence-H00003954-M03-img0005.jpg)
![Immunohistochemistry-Paraffin: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of monoclonal antibody to LETM1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/LETM1-Antibody-6F7-Immunohistochemistry-Paraffin-H00003954-M03-img0006.jpg)
![Western Blot: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of LETM1 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/LETM1-Antibody-6F7-Western-Blot-H00003954-M03-img0008.jpg)
![ELISA: LETM1 Antibody (6F7) [H00003954-M03] - Detection limit for recombinant GST tagged LETM1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LETM1-Antibody-6F7-ELISA-H00003954-M03-img0004.jpg)
![Immunoprecipitation: LETM1 Antibody (6F7) [H00003954-M03] - Analysis of LETM1 transfected lysate using anti-LETM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LETM1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/LETM1-Antibody-6F7-Immunoprecipitation-H00003954-M03-img0007.jpg)
![Western Blot: LETM1 Antibody [NBP1-33433] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/LETM1-Antibody-Western-Blot-NBP1-33433-img0004.jpg "Western Blot: LETM1 Antibody [NBP1-33433] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: LETM1 Antibody [NBP1-33433] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33433-img0002.jpg)
![Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-33433] - Mouse stomach. LETM1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunohistochemistry-Paraffin-NBP1-33433-img0008.jpg)
![Western Blot: LETM1 Antibody [NBP1-33433] - A431 lysates (Lane A), H1299 lysates (Lane B), Hela cells (Lane C) and Hep G2 lysates (Lane D) using NBP1-33433 at a dilution of 1:1000.](http://images.novusbio.com/fullsize/LETM1-Antibody-Western-Blot-NBP1-33433-img0001.jpg)
![Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-33433] - HSC3 xenograft, using LETM1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunohistochemistry-Paraffin-NBP1-33433-img0003.jpg)
![Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-33433] - Mouse colon. LETM1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunohistochemistry-Paraffin-NBP1-33433-img0009.jpg)
![Western Blot: LETM1 Antibody [NBP1-33433] - Deficiency or mutant PINK1 reduces phosphorylation of LETM1 at Thr192. a, b Proteins extracted from PINK1 WT or KO MEFs (a) or mouse brain (b) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. c Proteins extracted from human fibroblast of control (Con) or a PINK1-Q456X patient (Q456X) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. d HEK293 cells were transfected with Adtrack GFP control, AdPINK1-WT, & AdPINK1-Q456X mutant for 1 day. Endogenous LETM1 protein was isolated by IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. e SH-SY5Y cells were treated with 25 µM rotenone for 8 or 16 h. Total cell lysates were either analyzed by WB with anti-PINK1, or subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 antibodies by WB. All above experiments were replicated three times, respectively Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33433_rabbit-polyclonal-letm1-antibody-310202416161839.jpg)
![Western Blot: LETM1 Antibody [NBP1-33433] - Deficiency or mutant PINK1 reduces phosphorylation of LETM1 at Thr192. a, b Proteins extracted from PINK1 WT or KO MEFs (a) or mouse brain (b) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. c Proteins extracted from human fibroblast of control (Con) or a PINK1-Q456X patient (Q456X) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. d HEK293 cells were transfected with Adtrack GFP control, AdPINK1-WT, & AdPINK1-Q456X mutant for 1 day. Endogenous LETM1 protein was isolated by IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. e SH-SY5Y cells were treated with 25 µM rotenone for 8 or 16 h. Total cell lysates were either analyzed by WB with anti-PINK1, or subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 antibodies by WB. All above experiments were replicated three times, respectively Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33433_rabbit-polyclonal-letm1-antibody-31020241616041.jpg)
![Western Blot: LETM1 Antibody [NBP1-33433] - Deficiency or mutant PINK1 reduces phosphorylation of LETM1 at Thr192. a, b Proteins extracted from PINK1 WT or KO MEFs (a) or mouse brain (b) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. c Proteins extracted from human fibroblast of control (Con) or a PINK1-Q456X patient (Q456X) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. d HEK293 cells were transfected with Adtrack GFP control, AdPINK1-WT, & AdPINK1-Q456X mutant for 1 day. Endogenous LETM1 protein was isolated by IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. e SH-SY5Y cells were treated with 25 µM rotenone for 8 or 16 h. Total cell lysates were either analyzed by WB with anti-PINK1, or subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 antibodies by WB. All above experiments were replicated three times, respectively Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33433_rabbit-polyclonal-letm1-antibody-31020241618931.jpg)
![Western Blot: LETM1 Antibody [NBP1-33433] - Deficiency or mutant PINK1 reduces phosphorylation of LETM1 at Thr192. a, b Proteins extracted from PINK1 WT or KO MEFs (a) or mouse brain (b) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. c Proteins extracted from human fibroblast of control (Con) or a PINK1-Q456X patient (Q456X) were subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. d HEK293 cells were transfected with Adtrack GFP control, AdPINK1-WT, & AdPINK1-Q456X mutant for 1 day. Endogenous LETM1 protein was isolated by IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 by WB. e SH-SY5Y cells were treated with 25 µM rotenone for 8 or 16 h. Total cell lysates were either analyzed by WB with anti-PINK1, or subjected to IP with anti-LETM1, probed with pT192 & reprobed with anti-LETM1 antibodies by WB. All above experiments were replicated three times, respectively Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33433_rabbit-polyclonal-letm1-antibody-310202416163750.jpg)
![Western Blot: LETM1 Antibody [NBP1-33433] - The effect of PINK1-mediated phosphorylation of LETM1 on liposomes calcium release activity in vitro. a About 1 µg purified bacterial full-length His-LETM1-WT, T175E, & T192E were incorporated into liposomes & subjected to calcium release assay. Control (Con) indicates no proteins incorporated. b (top panel) Quantification of calcium release activity measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposome samples in a were analyzed by WB with anti-LETM1 antibody to show loading of proteins. c HEK293 cells were co-transfected with pAdtrack LETM1 (AdLETM1)-WT or T192A & Adtrack control GFP (AdGFP, WT, T192A) or PINK1 (AdPINK1, pWT, pT192A), & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG, probed with pT192 or anti-LETM1 by WB. The experiment was replicated three times. d HEK293 cells were co-transfected with AdLETM1-WT or T192A & AdGFP or AdPINK1, & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG & eluted by 3XFLAG peptide. The eluted proteins were subjected to calcium release assay using artificial liposomes. Control is without protein incorporated. e (top panel) Quantification of calcium release activity of d measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposomes samples in d were subjected to WB with anti-LETM1 antibody to show similar loading of proteins Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33433_rabbit-polyclonal-letm1-antibody-31020241618937.jpg)
![Western Blot: LETM1 Antibody [NBP1-33433] - The effect of PINK1-mediated phosphorylation of LETM1 on liposomes calcium release activity in vitro. a About 1 µg purified bacterial full-length His-LETM1-WT, T175E, & T192E were incorporated into liposomes & subjected to calcium release assay. Control (Con) indicates no proteins incorporated. b (top panel) Quantification of calcium release activity measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposome samples in a were analyzed by WB with anti-LETM1 antibody to show loading of proteins. c HEK293 cells were co-transfected with pAdtrack LETM1 (AdLETM1)-WT or T192A & Adtrack control GFP (AdGFP, WT, T192A) or PINK1 (AdPINK1, pWT, pT192A), & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG, probed with pT192 or anti-LETM1 by WB. The experiment was replicated three times. d HEK293 cells were co-transfected with AdLETM1-WT or T192A & AdGFP or AdPINK1, & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG & eluted by 3XFLAG peptide. The eluted proteins were subjected to calcium release assay using artificial liposomes. Control is without protein incorporated. e (top panel) Quantification of calcium release activity of d measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposomes samples in d were subjected to WB with anti-LETM1 antibody to show similar loading of proteins Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33433_rabbit-polyclonal-letm1-antibody-310202416163720.jpg)
![Western Blot: LETM1 Antibody [NBP1-33433] - The effect of PINK1-mediated phosphorylation of LETM1 on liposomes calcium release activity in vitro. a About 1 µg purified bacterial full-length His-LETM1-WT, T175E, & T192E were incorporated into liposomes & subjected to calcium release assay. Control (Con) indicates no proteins incorporated. b (top panel) Quantification of calcium release activity measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposome samples in a were analyzed by WB with anti-LETM1 antibody to show loading of proteins. c HEK293 cells were co-transfected with pAdtrack LETM1 (AdLETM1)-WT or T192A & Adtrack control GFP (AdGFP, WT, T192A) or PINK1 (AdPINK1, pWT, pT192A), & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG, probed with pT192 or anti-LETM1 by WB. The experiment was replicated three times. d HEK293 cells were co-transfected with AdLETM1-WT or T192A & AdGFP or AdPINK1, & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG & eluted by 3XFLAG peptide. The eluted proteins were subjected to calcium release assay using artificial liposomes. Control is without protein incorporated. e (top panel) Quantification of calcium release activity of d measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposomes samples in d were subjected to WB with anti-LETM1 antibody to show similar loading of proteins Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33433_rabbit-polyclonal-letm1-antibody-310202416175274.jpg)
![Western Blot: LETM1 Antibody [NBP1-33433] - The effect of PINK1-mediated phosphorylation of LETM1 on liposomes calcium release activity in vitro. a About 1 µg purified bacterial full-length His-LETM1-WT, T175E, & T192E were incorporated into liposomes & subjected to calcium release assay. Control (Con) indicates no proteins incorporated. b (top panel) Quantification of calcium release activity measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposome samples in a were analyzed by WB with anti-LETM1 antibody to show loading of proteins. c HEK293 cells were co-transfected with pAdtrack LETM1 (AdLETM1)-WT or T192A & Adtrack control GFP (AdGFP, WT, T192A) or PINK1 (AdPINK1, pWT, pT192A), & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG, probed with pT192 or anti-LETM1 by WB. The experiment was replicated three times. d HEK293 cells were co-transfected with AdLETM1-WT or T192A & AdGFP or AdPINK1, & AdLETM1-T192E only for 1 day. The FLAG-LETM1 proteins were isolated by IP with anti-FLAG & eluted by 3XFLAG peptide. The eluted proteins were subjected to calcium release assay using artificial liposomes. Control is without protein incorporated. e (top panel) Quantification of calcium release activity of d measured by rate of calcium release & calculated as change of fluorescence unit (∆F). n = 6. (bottom panel) The liposomes samples in d were subjected to WB with anti-LETM1 antibody to show similar loading of proteins Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29123128), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-33433_rabbit-polyclonal-letm1-antibody-310202416173535.jpg)
![Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556] - Staining in human colon and pancreas tissues using anti-LETM1 antibody. Corresponding LETM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunohistochemistry-Paraffin-NBP1-89556-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556] - Staining in human colon and pancreas tissues using anti-LETM1 antibody. Corresponding LETM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: LETM1 Antibody [NBP1-89556] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/LETM1-Antibody-Western-Blot-NBP1-89556-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: LETM1 Antibody [NBP1-89556] - Staining of human cell line U-251 MG shows localization to mitochondria.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89556-img0013.jpg)
![Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556] - Staining of human colon shows high expression.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunohistochemistry-Paraffin-NBP1-89556-img0010.jpg)
![Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-89556] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunohistochemistry-Paraffin-NBP1-89556-img0011.jpg)
![Simple Western: LETM1 Antibody [NBP1-89556] - Simple Western lane view shows a specific band for LETM1 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LETM1-Antibody-Simple-Western-NBP1-89556-img0006.jpg)
![Simple Western: LETM1 Antibody [NBP1-89556] - Electropherogram image(s) of corresponding Simple Western lane view. LETM1 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/LETM1-Antibody-Simple-Western-NBP1-89556-img0007.jpg)
![Western Blot: LETM1 Antibody (2C6) [H00003954-M02] - LETM1 monoclonal antibody (M02), clone 2C6. Analysis of LETM1 expression in HeLa.](http://images.novusbio.com/fullsize/LETM1-Antibody-2C6-Western-Blot-H00003954-M02-img0004.jpg "Western Blot: LETM1 Antibody (2C6) [H00003954-M02] - LETM1 monoclonal antibody (M02), clone 2C6. Analysis of LETM1 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: LETM1 Antibody (2C6) [H00003954-M02] - Analysis of monoclonal antibody to LETM1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/LETM1-Antibody-2C6-Immunocytochemistry-Immunofluorescence-H00003954-M02-img0002.jpg)
![Sandwich ELISA: LETM1 Antibody (2C6) [H00003954-M02] - Detection limit for recombinant GST tagged LETM1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/LETM1-Antibody-2C6-Sandwich-ELISA-H00003954-M02-img0003.jpg)
![Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-86482] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunohistochemistry-Paraffin-NBP1-86482-img0005.jpg "Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-86482] - Staining of human pancreas shows low expression as expected.")
![Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-86482] - Staining of human colon shows high expression.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunohistochemistry-Paraffin-NBP1-86482-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LETM1 Antibody [NBP1-86482] - Staining in human colon and pancreas tissues using anti-LETM1 antibody. Corresponding LETM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LETM1-Antibody-Immunohistochemistry-Paraffin-NBP1-86482-img0006.jpg)
![Western Blot: LETM1 Antibody [NBP1-59611] - Jurkat cell lysate, concentration 5.0ug/ml.](http://images.novusbio.com/fullsize/LETM1-Antibody-Western-Blot-NBP1-59611-img0002.jpg "Western Blot: LETM1 Antibody [NBP1-59611] - Jurkat cell lysate, concentration 5.0ug/ml.")