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WASF3/WAVE3 Antibody

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Western blot shows lysates of A172 human glioblastoma cell line, SH-SY5Y human neuroblastoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse WASF3/WAVE3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WASF3/WAVE3 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of A172 human glioblastoma cell line, SH-SY5Y human neuroblastoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse WASF3/WAVE3 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5515-SP
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WASF3/WAVE3 Antibody Summary

Immunogen
E. coli-derived recombinant human WASF3/WAVE3
Met1-Lys180
Accession # Q9UPY6
Specificity
Detects endogenous human/mouse WASF3/WAVE3 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
WASF3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5515 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for WASF3/WAVE3 Antibody

  • Brush-1
  • KIAA0900WASP family Verprolin-homologous protein 3
  • Protein WAVE-3
  • SCAR3
  • SCAR3WASP family protein member 3
  • Verprolin homology domain-containing protein 3
  • WAS protein family, member 3
  • WASF3
  • WAVE3
  • WAVE3Brush-1
  • wisk

Background

WASF3/WAVE3 (Wiscott-Aldrich syndrome protein family Verprolin-homologous protein 3) is a 70 kDa member of the SCAR/WAVE family of proteins. It is expressed in select diverse cell types such as neurons, platelets, and breast epithelium. Upon activation, WASF3/WAVE3 is phosphorylated on Tyr151/248/337/486 and, in complex with HSPC300, initiates actin polymerization. WASF3/WAVE3 also regulates MMP expression. Human WASF3/WAVE3 is 502 amino acids (aa) in length. It contains one coiled-coil region (aa 57‑93), three sequential poly-Pro regions (aa 304‑410) and one VPH domain (aa 440‑457) that binds to actin and Arp2/3. There is one isoform that shows a 36 aa substitution for aa 181‑239. Over aa 1‑180, human WASF3 shares 98% aa identity with mouse WASF3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for WASF3/WAVE3 Antibody (AF5515)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol WASF3
Uniprot