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MTTP Antibody

Images

 
Western Blot: MTTP Antibody [NBP1-62489] - Adult mouse small intestine homogenate, 20 ug total protein per lane. Antibody at 1:1000. Secondary HRP conjugated antibody at 1:5000. Bands visualized with Western ...read more
Western Blot: MTTP Antibody [NBP1-62489] - HepG2 cell lysate.

Product Details

Summary
Reactivity Hu, Mu, DrSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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MTTP Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptide directed towards the N terminal of human MTTP (NP_000244). Peptide sequence within the following region: MILLAVLFLCFISSYSASVKGHTTGLSLNNDRLYKLTYSTEVLLDRGKGK. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTTP
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-62489 in the following application:

Publications
Read Publication using NBP1-62489.

Reactivity Notes

Immunostaining on Drosophila third instar larvae has been published. Mouse reactivity reported in scientific literature (PMID: 29844386).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for MTTP Antibody

  • 88kD)
  • 88kDa)
  • ABL
  • MGC149819
  • microsomal triglyceride transfer protein large subunit
  • microsomal triglyceride transfer protein
  • MTPMGC149820

Background

MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia.MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MTTP Antibody (NBP1-62489)(1)

We have publications tested in 1 confirmed species: Mouse.


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Review for MTTP Antibody (NBP1-62489) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-62489:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot MTTP NBP1-62489
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5
reviewed by:
Verified Customer
WB Mouse 10/23/2019
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Summary

ApplicationWestern Blot
Sample TestedAdult small intestine
SpeciesMouse
LotQC15947-43641

Comments

CommentsMouse small intestine was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody Mttp diluted 1:1000 in 5% BSA 2, before incubation with anti rabbit secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.

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Video Protocols

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FAQs for MTTP Antibody (NBP1-62489) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for MTTP Antibody (NBP1-62489)

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Blogs on MTTP

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Recent Reviews

5
5
1
4
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3
0
2
0
1
0

Verified Customer
10/23/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MTTP
Entrez
Uniprot