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VPS36 Antibody

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Immunocytochemistry/ Immunofluorescence: VPS36 Antibody [NBP2-13518] - Staining of human cell line U-2 OS shows localization to vesicles & lysosomes.
Immunohistochemistry-Paraffin: VPS36 Antibody [NBP2-13518] - Staining of human pancreas shows weak cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: VPS36 Antibody [NBP2-13518] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: VPS36 Antibody [NBP2-13518] - Staining of human rectum shows moderate cytoplasmic postivity in glandular cells.
Immunohistochemistry-Paraffin: VPS36 Antibody [NBP2-13518] - Staining of human kidney shows weak cytoplasmic positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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VPS36 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: AGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRG
Predicted Species
Mouse (93%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VPS36
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
VPS36 Protein (NBP2-13518PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for VPS36 Antibody

  • C13orf9
  • CGI-145
  • DKFZp781E0871
  • Eap45
  • EAP45chromosome 13 open reading frame 9
  • ELL-associated protein of 45 kDa
  • ELL-associated protein, 45 kDa
  • ESCRT-II complex subunit VPS36
  • vacuolar protein sorting 36 homolog (S. cerevisiae)
  • vacuolar protein sorting 36 homolog (yeast)
  • vacuolar protein-sorting-associated protein 36

Background

Component of the escrt-ii complex, which is required for multivesicular bodies (mvbs) formation and sorting of endosomal cargo proteins into mvbs. the mvb pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. the escrt-ii complex is probably involved in the recruitment of the escrt-iii complex. its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by escrt complexes. the escrt-ii complex may also play a role in transcription regulation, possibly via its interaction with ell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol VPS36