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NPLOC4 Antibody

Images

 
Independent Antibodies: Western Blot: NPLOC4 Antibody [NBP1-82166] - Analysis using Anti-NPLOC4 antibody NBP1-82166 (A) shows similar pattern to independent antibody NBP1-82165 (B).
Immunocytochemistry/ Immunofluorescence: NPLOC4 Antibody [NBP1-82166] - Staining of human cell line A-431 shows positivity in nuclei but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Western Blot: NPLOC4 Antibody [NBP1-82166] - Analysis in human cell line A-549.
Western Blot: NPLOC4 Antibody [NBP1-82166] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Western Blot: NPLOC4 Antibody [NBP1-82166] - Whole cell lysate of PC12 using antibody against nuclear protein NPLOC4. Samples were run as triplicates. Image from verified customer review.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human placenta shows moderate granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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NPLOC4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPLOC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NPLOC4 Protein (NBP1-82166PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-82166 in the following applications:

Publications
Read Publications using
NBP1-82166 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NPLOC4 Antibody

  • FLJ20657
  • FLJ23742
  • KIAA1499nuclear protein localization protein 4 homolog
  • NPL4Protein NPL4
  • nuclear protein localization 4 homolog (S. cerevisiae)

Background

The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NPLOC4 Antibody (NBP1-82166)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IHC-P, WB, Western Blot.


Filter By Application
ICC/IF
(4)
IHC-P
(1)
WB
(5)
Western Blot
(1)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-82166 Applications Species
Kanellis DC, Zisi A, Skrott Z et al. Actionable cancer vulnerability due to translational arrest, p53 aggregation and ribosome biogenesis stress evoked by the disulfiram metabolite CuET Cell death and differentiation 2023-05-04 [PMID: 37142656] (ICC/IF, WB, Human) ICC/IF, WB Human
Chroma K, Skrott Z, Gursky J et al. A drug repurposing strategy for overcoming human multiple myeloma resistance to standard-of-care treatment Cell death & disease 2022-03-04 [PMID: 35246527] (WB, Human) WB Human
Serra R, Zhao T, Huq S Et al. Disulfiram and copper combination therapy targets NPL4, cancer stem cells and extends survival in a medulloblastoma model PloS one 2021-11-03 [PMID: 34731160] (ICC/IF, IHC-P, WB, Human) ICC/IF, IHC-P, WB Human
A Franz, P Valledor, P Ubieto-Cap, D Pilger, A Galarreta, V Lafarga, A Fernández-, G de la Vega, F den Brave, T Hoppe, O Fernandez-, E Lecona USP7 and VCPFAF1 define the SUMO/Ubiquitin landscape at the DNA replication fork Cell Reports, 2021-10-12;37(2):109819. 2021-10-12 [PMID: 34644576]
Buchtova T, Skrott Z, Chroma K et al. Cannabidiol-induced activation of the metallothionein pathway impedes anticancer effects of disulfiram and its metabolite CuET Molecular Oncology 2022-04-01 [PMID: 34632694] (Western Blot) Western Blot
Skrott Z, Mistrik M, Andersen KK et al. Alcohol-abuse drug disulfiram targets cancer via p97 segregase adaptor NPL4. Nature 2017-12-14 [PMID: 29211715] (WB, ICC/IF, Human) WB, ICC/IF Human
Davis EJ, Lachaud C, Appleton P et al. DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage. Nat Struct Mol Biol 2012-11-01 [PMID: 23042607]
Zhang H, Wang T, Liu H et al. Boost therapy of hepatocellular carcinoma by amplifying vicious cycle between mitochondrial oxidative stress and endoplasmic reticulum stress via biodegradable ultrasmall nanoparticles and old drug Nano Today 2022-10-01 (WB, ICC/IF, Human)

Details:
ICC/If Dilution: 1:200; WB Dilution: 1:1000
WB, ICC/IF Human

Review for NPLOC4 Antibody (NBP1-82166) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-82166:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot NPLOC4 NBP1-82166
Enlarge
5
reviewed by:
Luiz Alexandre Magno
WB Rat 10/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedPC12 cell lysate
SpeciesRat
LotA96635

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NPLOC4 Antibody (NBP1-82166) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Luiz Alexandre Magno
10/17/2018
Application: WB
Species: Rat

Bioinformatics

Gene Symbol NPLOC4