Home » VEGFR2/KDR/Flk-1 » VEGFR2/KDR/Flk-1 Antibodies » VEGFR2/KDR/Flk-1 Antibody (89109) [Unconjugated]
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VEGFR2/KDR/Flk-1 Antibody (89109) [Unconjugated]

 (6 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Cap)
Clone
89109
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3573-SP
Carrier Free
MAB3573-500
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VEGFR2/KDR/Flk-1 Antibody (89109) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR2/KDR/Flk-1
Ala20-Glu764
Accession # P35968
Specificity
Detects human VEGFR2/KDR/Flk-1 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) VEGFR1, rhVEGFR3, or recombinant mouse VEGFR2 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KDR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human VEGF R2/KDR/Flk-1 Biotinylated Antibody (Catalog number BAF357)
Standard: Recombinant Human VEGF R2/KDR/Flk-1 Fc Chimera (Catalog number 357-KD)
Publications
Read Publications using
MAB3573 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VEGFR2/KDR/Flk-1 Antibody (89109) [Unconjugated]

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGFR2 cDNA encodes a 1356 amino acid (aa) residue precursor protein with a 19 aa residue signal peptide. Mature VEGFR2 is composed of a 745 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 567 aa residue cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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VEGFR2/KDR/Flk-1
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MAB3573
VEGFR2/KDR/Flk-1 Antibody (89109) [Unconjugated]
Species: Hu
Applications: WB, ELISA(Cap)

Publications for VEGFR2/KDR/Flk-1 Antibody (MAB3573)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ELISA Development, Neutralization, Western Blot.


Filter By Application
ELISA Development
(1)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB3573 Applications Species
James P. Landry, Yiyan Fei, Xiangdong Zhu, Yaohuang Ke, Guoliang Yu, Pierre Lee Discovering Small Molecule Ligands of Vascular Endothelial Growth Factor That Block VEGF–KDR Binding Using Label-Free Microarray-Based Assays ASSAY and Drug Development Technologies 2013-06-19 [PMID: 23772553]
Stephan Niland, Bartosz Ditkowski, Désirée Parrandier, Lise Roth, Hellmut Augustin, Johannes A. Eble Rhodocetin-alpha beta –induced Neuropilin-1–cMet Association Triggers Restructuring of Matrix Contacts in Endothelial Cells Arteriosclerosis, Thrombosis, and Vascular Biology 2013-03-01 [PMID: 23288161]
Meissner M, Reichenbach G, Stein M, Hrgovic I, Kaufmann R, Gille J Down-regulation of vascular endothelial growth factor receptor 2 is a major molecular determinant of proteasome inhibitor-mediated antiangiogenic action in endothelial cells. Cancer Res., 2009-02-17;69(5):1976-84. 2009-02-17 [PMID: 19223539] (Western Blot, Human) Western Blot Human
Wen J, Fu AF, Chen LJ, Xie XJ, Yang GL, Chen XC, Wang YS, Li J, Chen P, Tang MH, Shao XM, Lu Y, Zhao X, Wei YQ Liposomal honokiol inhibits VEGF-D-induced lymphangiogenesis and metastasis in xenograft tumor model. Int. J. Cancer, 2009-06-01;124(11):2709-18. 2009-06-01 [PMID: 19219913] (Western Blot, Human) Western Blot Human
Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp. Hematol., 2008-06-17;36(9):1121-31. 2008-06-17 [PMID: 18562079] (Neutralization, Human) Neutralization Human
Oliner J, Min H, Leal J, Yu D, Rao S, You E, Tang X, Kim H, Meyer S, Han SJ, Hawkins N, Rosenfeld R, Davy E, Graham K, Jacobsen F, Stevenson S, Ho J, Chen Q, Hartmann T, Michaels M, Kelley M, Li L, Sitney K, Martin F, Sun JR, Zhang N, Lu J, Estrada J, Kumar R, Coxon A, Kaufman S, Pretorius J, Scully S, Cattley R, Payton M, Coats S, Nguyen L, Desilva B, Ndifor A, Hayward I, Radinsky R, Boone T, Kendall R Suppression of angiogenesis and tumor growth by selective inhibition of angiopoietin-2. Cancer Cell, 2004-11-01;6(5):507-16. 2004-11-01 [PMID: 15542434] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol KDR
Uniprot