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VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free

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Western Blot: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein. Image from the standard format of this antibody.
Immunocytochemistry: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Analysis of FFPE tissue section of Human Hepatocellular Carcinoma with antibody at 5 ug/ml concentration. The cancer cells ...read more
Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Analysis of FFPE tissue section of Human SCC of Esophagus with antibody at 5 ug/ml concentration. The cancer cells ...read more
Flow (Cell Surface): VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - HepG2 cells were stained (surface) with antibody (orange) or isotype control, mouse IgG1 (blue). Positive staining observed using ...read more
Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Antibody (1:50) was tested in HUVEC cells with DyLight 488 (green). Nuclei and alpha-tubulin were ...read more
Immunohistochemistry: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Aanalysis of FFPE transverse section of normal human skeletal muscle with antibody at 5 ug/ml concentration. The myocytes showed ...read more
Immunohistochemistry: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with antibody at 5 ug/ml concentration. The ...read more
Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free [NBP2-81009] - Analysis of FFPE tissue section of human normal kidney with antibody at 5 ug/ml concentration. The cuboidal epithelial ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
2C6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

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VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free Summary

Immunogen
Partial recombinant human VEGFR2 protein (between amino acids 300-600) [UniProt# P35968].
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KDR
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow (Cell Surface)
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for VEGFR2/KDR/Flk-1 Antibody (2C6) - Azide and BSA Free

  • CD309 antigen
  • CD309
  • EC 2.7.10
  • EC 2.7.10.1
  • Fetal liver kinase 1
  • fetal liver kinase-1
  • Flk1
  • Flk-1
  • FLK1tyrosine kinase growth factor receptor
  • KDR
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • Kinase insert domain receptor
  • KRD1
  • Ly73
  • Protein-tyrosine kinase receptor flk-1
  • soluble VEGFR2
  • vascular endothelial growth factor receptor 2
  • VEGF R2
  • VEGFR
  • VEGFR2
  • VEGFR-2

Background

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol KDR