![Western Blot: VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Analysis of recombinant human and mouse soluble VEGF receptors using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a cross reactivity with mouse sFlt-1(D7)-Fc but no with human and mouse sKDR and human sFLT-4 visible.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-Flt-1-EWC-Western-Blot-NB600-1004-img0004.jpg "Western Blot: VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Analysis of recombinant human and mouse soluble VEGF receptors using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a cross reactivity with mouse sFlt-1(D7)-Fc but no with human and mouse sKDR and human sFLT-4 visible.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Func, ICC/IF |
| Clone | EWC |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Recombinant human soluble extracellular Flt-1 (D5) |
| Localization | Type I membrane protein (Flt1) and soluble (SFlt1). |
| Marker | Endothelial Cell Marker |
| Specificity | The monoclonal will detect native and denaturated human VEGFR-1/ Flt-1 in ELISA experiments and on the surface of different human cell types. The will also detect mouse Flt-1. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FLT1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Use In vivo reported in scientific literature (PMID:31877123) |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Protein G purified |
| Reconstitution Instructions | Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form. |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
ELISA | Human | 11/01/2011 |
Summary
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reviewed by:
Zhiwei Zhou |
ELISA | Mouse | 09/23/2011 |
Summary
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reviewed by:
Verified Customer |
ELISA | Mouse | 09/16/2011 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for VEGFR1/Flt-1 Antibody (NB600-1004)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 11/01/2011 |
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| Application: | ELISA | |
| Species: | Human |
| Zhiwei Zhou 09/23/2011 |
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| Application: | ELISA | |
| Species: | Mouse |
| Verified Customer 09/16/2011 |
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| Application: | ELISA | |
| Species: | Mouse |
| Gene Symbol | FLT1 |
![Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free [NB600-1004] - VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Staining of human Flt-1 in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with a mouse anti-human Flt-1 antibody. Conjugated secondary antibody: goat anti-mouse ALEXA Flour (1:600).](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-Flt-1-EWC-Immunocytochemistry-Immunofluorescence-NB600-1004-img0007.jpg "Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody (EWC) - Azide and BSA Free [NB600-1004] - VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Staining of human Flt-1 in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with a mouse anti-human Flt-1 antibody. Conjugated secondary antibody: goat anti-mouse ALEXA Flour (1:600).")
![Western Blot: VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Analysis of recombinant mouse soluble VEGFR-1 conditioned supernatant using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a strong cross reactivity with the mouse sFlt-1 visible.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-Flt-1-EWC-Western-Blot-NB600-1004-img0006.jpg "Western Blot: VEGFR1/Flt-1 Antibody (Flt-1/EWC) [NB600-1004] - Analysis of recombinant mouse soluble VEGFR-1 conditioned supernatant using a monoclonal antibody directed against human recombinant sFlt-1(D5). There is a strong cross reactivity with the mouse sFlt-1 visible.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")