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VEGFR1/Flt-1 Antibody (EWC) [Biotin]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
EWC
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-1004AF350
Alexa Fluor 405
NB600-1004AF405
Alexa Fluor 488
NB600-1004AF488
Alexa Fluor 532
NB600-1004AF532
Alexa Fluor 594
NB600-1004AF594
Alexa Fluor 647
NB600-1004AF647
Alexa Fluor 700
NB600-1004AF700
Alexa Fluor 750
NB600-1004AF750
CoraFluor 1
NB600-1004CL1
DyLight 350
NB600-1004UV
DyLight 405
NB600-1004V
DyLight 488
NB600-1004G
DyLight 550
NB600-1004R
DyLight 594
NB600-1004DL594
DyLight 650
NB600-1004C
DyLight 680
NB600-1004FR
DyLight 755
NB600-1004IR
FITC
NB600-1004F
HRP
NB600-1004H
Janelia Fluor 525
NB600-1004JF525
Janelia Fluor 549
NB600-1004JF549
Janelia Fluor 646
NB600-1004JF646
Janelia Fluor 669
NB600-1004JF669
PerCP
NB600-1004PCP
Unconjugated
NB600-1004
mFluor Violet 450 SE
NB600-1004MFV450
mFluor Violet 500 SE
NB600-1004MFV500
mFluor Violet 610 SE
NB600-1004MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB600-1004
Azide and BSA Free
NB600-1004
Azide and BSA Free
NB600-1004-20ug
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VEGFR1/Flt-1 Antibody (EWC) [Biotin] Summary

Immunogen
Recombinant human soluble extracellular Flt-1 (D5)
Localization
Type I membrane protein (Flt1) and soluble (SFlt1).
Marker
Endothelial Cell Marker
Specificity
The monoclonal will detect native and denaturated human VEGFR-1/ Flt-1 in ELISA experiments and on the surface of different human cell types. The will also detect mouse Flt-1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FLT1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA 2.5 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 1-5 ug/ml
  • Western Blot 2-5 ug/ml
Application Notes
This is useful for the following applications: ELISA, Western blotting, immunofluorescence, and immunoprecipitation.
Readout System

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS; 50X BSA
Preservative
0.02% Sodium Azide
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Alternate Names for VEGFR1/Flt-1 Antibody (EWC) [Biotin]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FLT1