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VEGFR1/Flt-1 Antibody (EWC) [DyLight 755]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Func, ICC/IF
Clone
EWC
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 755

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-1004AF350
Alexa Fluor 405
NB600-1004AF405
Alexa Fluor 488
NB600-1004AF488
Alexa Fluor 532
NB600-1004AF532
Alexa Fluor 594
NB600-1004AF594
Alexa Fluor 647
NB600-1004AF647
Alexa Fluor 700
NB600-1004AF700
Alexa Fluor 750
NB600-1004AF750
Biotin
NBP1-18636
CoraFluor 1
NB600-1004CL1
DyLight 350
NB600-1004UV
DyLight 405
NB600-1004V
DyLight 488
NB600-1004G
DyLight 550
NB600-1004R
DyLight 594
NB600-1004DL594
DyLight 650
NB600-1004C
DyLight 680
NB600-1004FR
FITC
NB600-1004F
HRP
NB600-1004H
Janelia Fluor 525
NB600-1004JF525
Janelia Fluor 549
NB600-1004JF549
Janelia Fluor 585
NB600-1004JF585
Janelia Fluor 635
NB600-1004JF635
Janelia Fluor 646
NB600-1004JF646
Janelia Fluor 669
NB600-1004JF669
PerCP
NB600-1004PCP
Unconjugated
NB600-1004
mFluor Violet 450 SE
NB600-1004MFV450
mFluor Violet 500 SE
NB600-1004MFV500
mFluor Violet 610 SE
NB600-1004MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB600-1004
Azide and BSA Free
NB600-1004
Azide and BSA Free
NB600-1004-20ug
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VEGFR1/Flt-1 Antibody (EWC) [DyLight 755] Summary

Immunogen
Recombinant human soluble extracellular Flt-1 (D5)
Localization
Type I membrane protein (Flt1) and soluble (SFlt1).
Marker
Endothelial Cell Marker
Specificity
The monoclonal will detect native and denaturated human VEGFR-1/ Flt-1 in ELISA experiments and on the surface of different human cell types. The will also detect mouse Flt-1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FLT1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • In vivo assay
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for VEGFR1/Flt-1 Antibody (EWC) [DyLight 755]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT
  • FLT1
  • Flt-1
  • Fms-like tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor)
  • FRT
  • Tyrosine-protein kinase FRT
  • Tyrosine-protein kinase receptor FLT
  • vascular endothelial growth factor receptor 1
  • Vascular permeability factor receptor
  • VEGF R1
  • VEGFR1
  • VEGFR-1

Background

Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FLT1