Immunocytochemistry/ Immunofluorescence: VEGF-C Antibody [NB110-61022] - bEnd.3 mouse brain endothelioma cell line stained with Vegf-c antibody at 1:500. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: VEGF-C Antibody [NB110-61022] - Pterostilbene significantly inhibits MTA1-dependent cell proliferation and angiogenesis and induces MTA1-targeted apoptosis in Ptenf/f mice(A) ...read more
Immunohistochemistry: VEGF-C Antibody [NB110-61022] - VEGFC Antibody [NB110-61022] - 4% PFA fixed and paraffin embedded mouse Lung tissue section was subjected to IHC staining of mouse VEGF-C.
Western Blot: VEGF-C Antibody [NB110-61022] - Kallikrein-related peptidase 3 (KLK3)/Prostate specific antigen (PSA) activates VEGF-C.(A, B) Cleavage of pro-VEGF-C by KLK3 (PSA). Pro-VEGF-C was incubated with or without ...read more
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with sterilized PBS to a final concentration of 0.2 mg/mL.
Alternate Names for VEGF-C Antibody
Flt4 ligand
Flt4-L
vascular endothelial growth factor C
Vascular endothelial growth factor-related protein
VEGFC
VEGF-C
VRPFLT4 ligand DHM
Background
Vascular endothelial growth factors (VEGFs), also known as vasculotropins, are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. The target cell specificity of VEGF is restricted to vascular endothelial cells. Vascular Endothelial Growth Factor C (VEGFC) is a member of the VEGF subfamily of PDGF-related growth factors. It is the ligand for Flt4 (VEGFR3) and KDR (VEGFR2). VEGFC binds Flt4 and induces tyrosine autophosphorylation of VEGFR3 and VEGFR2. VEGFC also stimulates the migration of bovine capillary endothelial cells in collagen gel. It is a specific growth factor for the lymphatic vascular system and mediates lymphangiogenesis. VEGFC is abundantly expressed in heart and skeletal muscle. Other tissues such as lung and kidney also express VEGFC. The human VEGFC gene is located on chromosome 4q34.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Travisano S, Harrison M, Thornton M et al. Single nuclei multiomic analyses identify human cardiac lymphatic endothelial cells associated with coronary arteries in the epicardium bioRxiv 2023-04-02 (IHC)
FAQs for VEGF-C Antibody (NB110-61022). (Showing 1 - 3 of 3 FAQs).
I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
What is the molecular weight for VEGFC Antibody (NB110-61022) on Western blot?
The expected molecular weight of VEGFC is about 47KDa according to UniProt.
Do you have any data which show the molecular weight of the VEGFC antibody (NB110-61022) by western blotting?
We do not currently have any images of NB110-61022 being used in Western blot, although it is guaranteed for this use. NBP1-45924 and NBP1-97842 show a band around 16 kDa. This is the predicted molecular weight of VEGF-C (UniProt P49767) and this is the weight the band using NB110-61022 should show up at.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: VEGF-C Antibody [NB110-61022] - bEnd.3 mouse brain endothelioma cell line stained with Vegf-c antibody at 1:500. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/VEGF-C-Antibody-Immunocytochemistry-Immunofluorescence-NB110-61022-img0002.jpg "Immunocytochemistry/Immunofluorescence: VEGF-C Antibody [NB110-61022] - bEnd.3 mouse brain endothelioma cell line stained with Vegf-c antibody at 1:500. ICC/IF image submitted by a verified customer review.")
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![Immunohistochemistry VEGFR2/KDR/Flk-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/VEGF_R2_AF644_Immunohistochemistry_22159.jpg)
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![Immunohistochemistry-Paraffin: VEGF-C Antibody [NB110-61022] - Pterostilbene significantly inhibits MTA1-dependent cell proliferation and angiogenesis and induces MTA1-targeted apoptosis in Ptenf/f mice(A) Representative Ki-67 (top, each panel), CD31 (middle, each panel) and VEGF-C (bottom, each panel) staining of the prostate tissues from Ptenf/f mice treated with vehicle and PTER, at indicated ages. Arrows indicate vessels. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/7841) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VEGF-C-Antibody-Immunohistochemistry-Paraffin-NB110-61022-img0003.jpg "Immunohistochemistry-Paraffin: VEGF-C Antibody [NB110-61022] - Pterostilbene significantly inhibits MTA1-dependent cell proliferation and angiogenesis and induces MTA1-targeted apoptosis in Ptenf/f mice(A) Representative Ki-67 (top, each panel), CD31 (middle, each panel) and VEGF-C (bottom, each panel) staining of the prostate tissues from Ptenf/f mice treated with vehicle and PTER, at indicated ages. Arrows indicate vessels. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/7841) licensed under a CC-BY license.")
![Immunohistochemistry: VEGF-C Antibody [NB110-61022] - VEGFC Antibody [NB110-61022] - 4% PFA fixed and paraffin embedded mouse Lung tissue section was subjected to IHC staining of mouse VEGF-C.](http://images.novusbio.com/fullsize/VEGF-C-Antibody-Immunohistochemistry-NB110-61022-img0001.jpg "Immunohistochemistry: VEGF-C Antibody [NB110-61022] - VEGFC Antibody [NB110-61022] - 4% PFA fixed and paraffin embedded mouse Lung tissue section was subjected to IHC staining of mouse VEGF-C.")
![Western Blot: VEGF-C Antibody [NB110-61022] - Kallikrein-related peptidase 3 (KLK3)/Prostate specific antigen (PSA) activates VEGF-C.(A, B) Cleavage of pro-VEGF-C by KLK3 (PSA). Pro-VEGF-C was incubated with or without KLK3, with & without the monoclonal antibody against KLK3 (5C7). Detection of VEGF-C in Western blots probed with antiserum 6 & 3/4, resulting in the detection of pro-VEGF-C (29/31 kDa) & activated, mature VEGF-C (21/23 kDa). The band marked by the asterisk likely represents the N-terminal propeptide (~15 kDa) which is detected by the antiserum 6. Note that for the image shown for antiserum 6, two different exposures of the same blot were merged (n = 3). (C, D) VEGF-C processed by KLK3 is biologically active in Ba/F3 cell assays, which translate activation of a hybrid VEGFR/EpoR receptor into cell survival (n = 2). Error bars indicate SD.10.7554/eLife.44478.004Figure 1—source data 1.Ba/F3 assay showing the activity of KLK3-cleaved VEGF-C.Ba/F3 assay showing the activity of KLK3-cleaved VEGF-C. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31099754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-61022_rabbit-polyclonal-vegf-c-antibody-31020241620375.jpg "Western Blot: VEGF-C Antibody [NB110-61022] - Kallikrein-related peptidase 3 (KLK3)/Prostate specific antigen (PSA) activates VEGF-C.(A, B) Cleavage of pro-VEGF-C by KLK3 (PSA). Pro-VEGF-C was incubated with or without KLK3, with & without the monoclonal antibody against KLK3 (5C7). Detection of VEGF-C in Western blots probed with antiserum 6 & 3/4, resulting in the detection of pro-VEGF-C (29/31 kDa) & activated, mature VEGF-C (21/23 kDa). The band marked by the asterisk likely represents the N-terminal propeptide (~15 kDa) which is detected by the antiserum 6. Note that for the image shown for antiserum 6, two different exposures of the same blot were merged (n = 3). (C, D) VEGF-C processed by KLK3 is biologically active in Ba/F3 cell assays, which translate activation of a hybrid VEGFR/EpoR receptor into cell survival (n = 2). Error bars indicate SD.10.7554/eLife.44478.004Figure 1—source data 1.Ba/F3 assay showing the activity of KLK3-cleaved VEGF-C.Ba/F3 assay showing the activity of KLK3-cleaved VEGF-C. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31099754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![N/A VEGF-D [HRP]](https://images.novusbio.com/images2/DATA_VEGFD_DVED00_ELISA_875.jpg)
![N/A VEGF-D [HRP]](https://images.novusbio.com/images2/DATA_VEGFD_DVED00_ELISA_876.jpg)
![N/A VEGF-D [HRP]](https://images.novusbio.com/images2/VEGF-D_DVED00_ELISA_210.jpg)
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![N/A PLGF [HRP]](https://images.novusbio.com/images2/DATA_PlGF_DPG00_ELISA_788.jpg)
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