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VCAM-1/CD106 Antibody [Unconjugated]

 (12 Publications)
 (1 Review)
 
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Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/ml Recombinant Human TNF‑ alpha (210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for VCAM‑1/CD106 at approximately 132 kDa (as indicated) using 20 µg/mL of Goat Anti-Human VCAM‑1/CD106 Polyclonal Antibody (Catalog # BBA19) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 10 ng/ml Recombinant Human TNF‑ alpha (210-TA) for 24 hrs, loaded at 0.2 mg/mL. A specific ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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VCAM-1/CD106 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human VCAM-1
Specificity
Detects VCAM‑1/CD106 in Western blots. VCAM‑1/CD106 has been screened using CHO cells transfected with cDNAs for ICAM-1 and VCAM-1.
Source
N/A
Isotype
Serum
Clonality
Polyclonal
Host
Goat
Gene
VCAM1
Purity Statement
N/A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 dilution
  • Simple Western 20 ug/mL
  • Western Blot 1:1000 dilution
Reviewed Applications
Read 1 Review rated 5
using
BBA19 in the following applications:
Publications
Read Publications using
BBA19 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Serum.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute in 0.5 mL of sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VCAM-1/CD106 Antibody [Unconjugated]

  • CD106 antigen
  • CD106
  • DKFZp779G2333
  • INCAM-100
  • L1CAM
  • MGC99561
  • vascular cell adhesion molecule 1
  • vascular cell adhesion protein 1
  • V-CAM 1
  • VCAM1
  • VCAM-1

Background

Vascular cell adhesion molecule (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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VCAM-1/CD106
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BBA19
VCAM-1/CD106 Antibody [Unconjugated]
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Applications: WB, Simple Western, IHC

Publications for VCAM-1/CD106 Antibody (BBA19)(12)

We have publications tested in 2 confirmed species: Human, Rabbit.

We have publications tested in 4 applications: ICC, IHC, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IHC
(1)
IHC-P
(1)
Western Blot
(4)
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Human
(6)
Rabbit
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using BBA19 Applications Species
Vasconcelos D, FalcAo L, da Ponte L et al. New Insights into the Mechanism of Immune-Mediated Tissue Injury in Yellow Fever: The Role of Immunopathological and Endothelial Alterations in the Human Lung Parenchyma Viruses 10/27/2022 [PMID: 36366477]
Alves Olimpio F, Magno FalcAo L, Gaia Carvalho M et al. Endothelium Activation during Severe Yellow Fever Triggers an Intense Cytokine-Mediated Inflammatory Response in the Liver Parenchyma Preprints 12/28/2021 [PMID: 35056050]
Interleukin-1 alpha Is a Central Regulator of Leukocyte-Endothelial Adhesion in Myocardial Infarction and in Chronic Kidney Disease [PMID: 34192892] (Western Blot, Human) Western Blot Human
AMS Barron, JC Mantero, JD Ho, B Nazari, KL Horback, J Bhawan, R Lafyatis, C Lam, JL Browning Perivascular Adventitial Fibroblast Specialization Accompanies T Cell Retention in the Inflamed Human Dermis J. Immunol., 2018-12-03;0(0):. 2018-12-03 [PMID: 30510068] (IHC, Human) IHC Human
Vladimir V. Shuvaev, Silvia Muro, Evguenia Arguiri, Makan Khoshnejad, Samira Tliba, Melpo Christofidou-Solomidou et al. Size and targeting to PECAM vs ICAM control endothelial delivery, internalization and protective effect of multimolecular SOD conjugates Journal of Controlled Release 7/1/2016 [PMID: 27210108]
Beatriz Marcos-Ramiro, Diego García-Weber, Susana Barroso, Jorge Feito, María C. Ortega, Eva Cernuda-Morollón et al. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border Journal of Cell Biology 5/9/2016 [PMID: 27138256]
Coiro P, Stoenica L, Strauss U, Brauer A Plasticity-related gene 5 promotes spine formation in murine hippocampal neurons. J Biol Chem, 2014-07-29;289(36):24956-70. 2014-07-29 [PMID: 25074937]
Kim J, Yoon Y, Lee J, Choi E, Yi N, Park K, Park J, Lotvall J, Kim Y, Gho Y Outer membrane vesicles derived from Escherichia coli up-regulate expression of endothelial cell adhesion molecules in vitro and in vivo. PLoS ONE, 2013-03-14;8(3):e59276. 2013-03-14 [PMID: 23516621] (Western Blot, Human) Western Blot Human
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig. Dis. Sci., 2007-03-30;52(10):2775-83. 2007-03-30 [PMID: 17394066] (ICC, Human) ICC Human
Miller AM, McPhaden AR, Preston A, Wadsworth RM, Wainwright CL TNFalpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation. Atherosclerosis, 2004-12-23;179(1):51-9. 2004-12-23 [PMID: 15721009] (IHC-P, Rabbit) IHC-P Rabbit
Show All 12 Publications.

Review for VCAM-1/CD106 Antibody (BBA19) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using BBA19:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB Human 01/20/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23774042
SpeciesHuman

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 Verified Customer
01/20/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol VCAM1