VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free Summary
Immunogen |
Purified recombinant fragment of human VCAM-1 expressed in E. coli. [Uniprot: P19320] |
Localization |
Membrane; Single-pass type I membrane protein. |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
VCAM1 |
Purity |
Ammonium sulfate precipitation |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:10000
- Immunoblotting reported in scientific literature (PMID 28569748)
- Immunohistochemistry 1:200-1:1000
- Immunohistochemistry-Paraffin 1:200-1:1000
- Immunoprecipitation reported in scientific literature (PMID 28569748)
- Simple Western 1:1000
- Western Blot 1:500-1:2000
|
Application Notes |
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
Theoretical MW |
81 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Ammonium sulfate precipitation |
Alternate Names for VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free
Background
VCAM1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. Tissue specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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