Novus Biologicals products are now on bio-techne.com

VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free

Images

 
Western Blot: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005] - Using VCAM1 mouse mAb against (1) HUVEC and (2) EC cell lysate. Image from the standard format of this antibody.
Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005] - Human placenta tissues using VCAM1 mouse mAb. Image from the standard format of this antibody.
Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005] - Breast carcinoma, cytoplasmic staining. Image from verified customer review. Image from the standard format of this antibody.
Immunohistochemistry: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005] - (A) human lung cancer and (B) colon cancer using VCAM1 mouse mAb with DAB staining. Image from the standard format of this antibody.
Simple Western: VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free [NBP2-81005] - Lane view shows a specific band for CD106/VCAM1 in 0.5 mg/ml of HUVEC lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ELISA, IHC, IP, WB
Clone
6G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free Summary

Immunogen
Purified recombinant fragment of human VCAM-1 expressed in E. coli. [Uniprot: P19320]
Localization
Membrane; Single-pass type I membrane protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
VCAM1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunoblotting reported in scientific literature (PMID 28569748)
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation reported in scientific literature (PMID 28569748)
  • Simple Western 1:1000
  • Western Blot 1:500-1:2000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free

  • CD106 antigen
  • CD106
  • DKFZp779G2333
  • INCAM-100
  • L1CAM
  • MGC99561
  • vascular cell adhesion molecule 1
  • vascular cell adhesion protein 1
  • V-CAM 1
  • VCAM1
  • VCAM-1

Background

VCAM1 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. Tissue specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF796
Species: Mu
Applications: AdBlk, IHC, WB
BBA16
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, WB
M6000B
Species: Mu
Applications: ELISA
7268-CT
Species: Hu
Applications: BA
DCP00
Species: Hu
Applications: ELISA
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF3628
Species: Hu, Mu, Rt
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
BBA10
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, Simple Western, WB
137-PS
Species: Hu
Applications: BA
AF1730
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, ICC
MAB3595
Species: Hu
Applications: CyTOF-ready, Flow, Simple Western, WB
MAB17781
Species: Hu
Applications: Block, CyTOF-ready, Flow, ICC, IP, KO
DY1707
Species: Hu
Applications: ELISA
AF4117
Species: Rt
Applications: IHC, WB
AF1320
Species: Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NB110-41083
Species: Hu
Applications: ChIP, ELISA, ICC/IF, WB
DVE00
Species: Hu
Applications: ELISA
NBP2-81005
Species: Hu, Mu
Applications: WB, Simple Western, ELISA, IHC, IP, WB

Publications for VCAM-1/CD106 Antibody (NBP2-81005) (0)

There are no publications for VCAM-1/CD106 Antibody (NBP2-81005).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for VCAM-1/CD106 Antibody (NBP2-81005) (0)

There are no reviews for VCAM-1/CD106 Antibody (NBP2-81005). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for VCAM-1/CD106 Antibody (NBP2-81005) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VCAM-1/CD106 Antibody (6G9) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol VCAM1