UNC13D/Munc 13-4 Antibody

Images

 
Western Blot: UNC13D/Munc 13-4 Antibody [NBP2-57827] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: UNC13D/Munc 13-4 Antibody [NBP2-57827] - Staining of human cell line U-2 OS shows localization to cytosol.
Orthogonal Strategies: Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827] - Staining in human spleen and kidney tissues using anti-UNC13D antibody. Corresponding UNC13D RNA-seq data are ...read more
Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827] - Staining of human kidney shows low expression as expected.
Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NBP2-57827] - Staining of human spleen shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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UNC13D/Munc 13-4 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SFHLDMWDLDTVESVRQKLGELTDLHGLRRIFKEARKDKGQDDFLGNVVLRLQDLRCREDQWYPLEPRTETYPDRGQCHLQFQLIHKRRATSASRSQPSYTVHLHLLQQLVSHEVTQHEAGSTSWDGSLSPQAATVLFLHATQKDLSD
Predicted Species
Mouse (94%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UNC13D
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
UNC13D/Munc 13-4 Recombinant Protein Antigen (NBP2-57827PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for UNC13D/Munc 13-4 Antibody

  • FHL3
  • HLH3
  • HPLH3
  • Munc13-4
  • Munc13-4protein unc-13 homolog D
  • unc-13 homolog D (C. elegans)
  • UNC13D

Background

Munc 13-4 encodes a protein that is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis type 3, a genetically heterogeneous, rare autosomal recessive disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UNC13D/Munc 13-4 Antibody (NBP2-57827) (0)

There are no publications for UNC13D/Munc 13-4 Antibody (NBP2-57827).
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UNC13D/Munc 13-4 Antibody (NBP2-57827). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking Munc13-4 N terminal antibody with related epitope sequence.
    • Unfortunately we do not epitope map our antibodies, but we do have a whole list of antibodies against your target depending on species reactivity and application you want to use them for. Please see this link to our MUNC 13-4 antibodies. All of the immunogen information that is not proprietary, or known should be presented on the datasheet. Often times a range will be provided where the peptide falls within, or if it was raised against a larger recombinant protein as is the case for the one you inquired about we do not know exactly where the binding occurs.
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Bioinformatics

Gene Symbol UNC13D