![Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - RBL-2H3 from Wt or shRNA Munc13-4 lentiviral stable expression whole cell lysate. 50 ug total protein. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Western-Blot-NB100-41385-img0008.jpg "Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - RBL-2H3 from Wt or shRNA Munc13-4 lentiviral stable expression whole cell lysate. 50 ug total protein. WB image submitted by a verified customer review.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-ETQKHKKDLHPLFD corresponding to internal region according to NP_954712.1. |
| Predicted Species | Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | UNC13D |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | WB: Approx. 105 kDa band observed in human T-lymphocyte and HeLa lysates (calculated MW of 123 kDa band according to NP_954712.1). IHC-P: Human thymus shows cytoplasm staining in select areas. Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out, provided by Micheal Tuvim, University of Texas MD Anderson Cancer Center, Houston, USA. Use in ICC/IF reported in scientific literature (PMID 28100639). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Scott Messenger |
WB | Rat | 04/14/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | UNC13D |
| Entrez | |
| Uniprot |
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![Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Mouse Lung with antibody at 0.5 ug/mL (wt in A and KO in B).](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Immunohistochemistry-Paraffin-NB100-41385-img0006.jpg "Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Mouse Lung with antibody at 0.5 ug/mL (wt in A and KO in B).")
![Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of Human T-lymphocyte lysate with antibody at 2 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Western-Blot-NB100-41385-img0007.jpg "Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of Human T-lymphocyte lysate with antibody at 2 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Human Thymus. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Immunohistochemistry-Paraffin-NB100-41385-img0005.jpg "Immunohistochemistry-Paraffin: UNC13D/Munc 13-4 Antibody [NB100-41385] - Staining of paraffin embedded Human Thymus. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA were left untreated (Untr) or were treated with TGFbeta-1 for 24 h, and SDS-PAGE Western blotting for indicated proteins was conducted. Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Knockdown-Validated-NB100-41385-img0009.jpg "Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA were left untreated (Untr) or were treated with TGFbeta-1 for 24 h, and SDS-PAGE Western blotting for indicated proteins was conducted. Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY license.")
![Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/UNC13D-Munc-13-4-Antibody-Knockdown-Validated-NB100-41385-img0010.jpg "Knockdown Validated: UNC13D/Munc 13-4 Antibody [NB100-41385] - SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). Image collected and cropped by CiteAb from the following publication (jcb.org/lookup/doi/10.1083/jcb.201710132), licensed under a CC-BY license.")
![Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Munc13-4 KD strongly impairs exosome release. (A) SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). (B) Culture medium from MDA-MB-231 cells either untreated or stimulated w/ 1.25 µM ionomycin for 30 min centrifuged at 1,000 g to remove cellular debris & 10,000 g to remove large extracellular vesicles. (C) The resulting 10,000-g supernatant filtered onto a nitrocellulose membrane & analyzed for CD63, CD9, ALIX, & GM130 content by antibody blotting. (D) Quantification of CD63, CD9, & ALIX blots in C are shown as exosome release as a percentage of total cellular material w/ mean values ± standard error (SE) for n ≥ 3. *, P < 0.05 for comparison w/ corresponding control samples. (E) Panc-1 or A549 cells left untreated or treated w/ TGF beta -1 for 24 h. Indicated proteins detected by SDS-PAGE Western blot. (F) Panc-1 cells left untreated or treated w/ TGF beta -1 for 24 h, & Munc13-4 levels determined by immunofluorescence. TGF beta -1–treated cells exhibited a mesenchymal morphology. Bars, 5 μm. (G) A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA left untreated (Untr) or treated w/ TGF beta -1 for 24 h, & SDS-PAGE Western blotting for indicated proteins conducted. (H) Culture media supernatants (as in B) from A549 cells that either untreated or stimulated w/ 1.25 µM ionomycin for 30 min filtered onto nitrocellulose membrane & analyzed for CD63 & GM130. (I) Quantification of CD63+ exosome release shown as a percentage of total cellular material w/ mean values ± SE for n = 5. *, P < 0.05; **, P < 0.01 for comparison w/ corresponding control samples. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29930202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41385_goat-polyclonal-unc13d-munc-13-4-antibody-3102024168395.jpg "Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Munc13-4 KD strongly impairs exosome release. (A) SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). (B) Culture medium from MDA-MB-231 cells either untreated or stimulated w/ 1.25 µM ionomycin for 30 min centrifuged at 1,000 g to remove cellular debris & 10,000 g to remove large extracellular vesicles. (C) The resulting 10,000-g supernatant filtered onto a nitrocellulose membrane & analyzed for CD63, CD9, ALIX, & GM130 content by antibody blotting. (D) Quantification of CD63, CD9, & ALIX blots in C are shown as exosome release as a percentage of total cellular material w/ mean values ± standard error (SE) for n ≥ 3. *, P < 0.05 for comparison w/ corresponding control samples. (E) Panc-1 or A549 cells left untreated or treated w/ TGF beta -1 for 24 h. Indicated proteins detected by SDS-PAGE Western blot. (F) Panc-1 cells left untreated or treated w/ TGF beta -1 for 24 h, & Munc13-4 levels determined by immunofluorescence. TGF beta -1–treated cells exhibited a mesenchymal morphology. Bars, 5 μm. (G) A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA left untreated (Untr) or treated w/ TGF beta -1 for 24 h, & SDS-PAGE Western blotting for indicated proteins conducted. (H) Culture media supernatants (as in B) from A549 cells that either untreated or stimulated w/ 1.25 µM ionomycin for 30 min filtered onto nitrocellulose membrane & analyzed for CD63 & GM130. (I) Quantification of CD63+ exosome release shown as a percentage of total cellular material w/ mean values ± SE for n = 5. *, P < 0.05; **, P < 0.01 for comparison w/ corresponding control samples. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29930202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Munc13-4 KD strongly impairs exosome release. (A) SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). (B) Culture medium from MDA-MB-231 cells either untreated or stimulated w/ 1.25 µM ionomycin for 30 min centrifuged at 1,000 g to remove cellular debris & 10,000 g to remove large extracellular vesicles. (C) The resulting 10,000-g supernatant filtered onto a nitrocellulose membrane & analyzed for CD63, CD9, ALIX, & GM130 content by antibody blotting. (D) Quantification of CD63, CD9, & ALIX blots in C are shown as exosome release as a percentage of total cellular material w/ mean values ± standard error (SE) for n ≥ 3. *, P < 0.05 for comparison w/ corresponding control samples. (E) Panc-1 or A549 cells left untreated or treated w/ TGF beta -1 for 24 h. Indicated proteins detected by SDS-PAGE Western blot. (F) Panc-1 cells left untreated or treated w/ TGF beta -1 for 24 h, & Munc13-4 levels determined by immunofluorescence. TGF beta -1–treated cells exhibited a mesenchymal morphology. Bars, 5 μm. (G) A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA left untreated (Untr) or treated w/ TGF beta -1 for 24 h, & SDS-PAGE Western blotting for indicated proteins conducted. (H) Culture media supernatants (as in B) from A549 cells that either untreated or stimulated w/ 1.25 µM ionomycin for 30 min filtered onto nitrocellulose membrane & analyzed for CD63 & GM130. (I) Quantification of CD63+ exosome release shown as a percentage of total cellular material w/ mean values ± SE for n = 5. *, P < 0.05; **, P < 0.01 for comparison w/ corresponding control samples. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29930202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41385_goat-polyclonal-unc13d-munc-13-4-antibody-310202415523941.jpg "Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Munc13-4 KD strongly impairs exosome release. (A) SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). (B) Culture medium from MDA-MB-231 cells either untreated or stimulated w/ 1.25 µM ionomycin for 30 min centrifuged at 1,000 g to remove cellular debris & 10,000 g to remove large extracellular vesicles. (C) The resulting 10,000-g supernatant filtered onto a nitrocellulose membrane & analyzed for CD63, CD9, ALIX, & GM130 content by antibody blotting. (D) Quantification of CD63, CD9, & ALIX blots in C are shown as exosome release as a percentage of total cellular material w/ mean values ± standard error (SE) for n ≥ 3. *, P < 0.05 for comparison w/ corresponding control samples. (E) Panc-1 or A549 cells left untreated or treated w/ TGF beta -1 for 24 h. Indicated proteins detected by SDS-PAGE Western blot. (F) Panc-1 cells left untreated or treated w/ TGF beta -1 for 24 h, & Munc13-4 levels determined by immunofluorescence. TGF beta -1–treated cells exhibited a mesenchymal morphology. Bars, 5 μm. (G) A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA left untreated (Untr) or treated w/ TGF beta -1 for 24 h, & SDS-PAGE Western blotting for indicated proteins conducted. (H) Culture media supernatants (as in B) from A549 cells that either untreated or stimulated w/ 1.25 µM ionomycin for 30 min filtered onto nitrocellulose membrane & analyzed for CD63 & GM130. (I) Quantification of CD63+ exosome release shown as a percentage of total cellular material w/ mean values ± SE for n = 5. *, P < 0.05; **, P < 0.01 for comparison w/ corresponding control samples. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29930202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Munc13-4 KD strongly impairs exosome release. (A) SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). (B) Culture medium from MDA-MB-231 cells either untreated or stimulated w/ 1.25 µM ionomycin for 30 min centrifuged at 1,000 g to remove cellular debris & 10,000 g to remove large extracellular vesicles. (C) The resulting 10,000-g supernatant filtered onto a nitrocellulose membrane & analyzed for CD63, CD9, ALIX, & GM130 content by antibody blotting. (D) Quantification of CD63, CD9, & ALIX blots in C are shown as exosome release as a percentage of total cellular material w/ mean values ± standard error (SE) for n ≥ 3. *, P < 0.05 for comparison w/ corresponding control samples. (E) Panc-1 or A549 cells left untreated or treated w/ TGF beta -1 for 24 h. Indicated proteins detected by SDS-PAGE Western blot. (F) Panc-1 cells left untreated or treated w/ TGF beta -1 for 24 h, & Munc13-4 levels determined by immunofluorescence. TGF beta -1–treated cells exhibited a mesenchymal morphology. Bars, 5 μm. (G) A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA left untreated (Untr) or treated w/ TGF beta -1 for 24 h, & SDS-PAGE Western blotting for indicated proteins conducted. (H) Culture media supernatants (as in B) from A549 cells that either untreated or stimulated w/ 1.25 µM ionomycin for 30 min filtered onto nitrocellulose membrane & analyzed for CD63 & GM130. (I) Quantification of CD63+ exosome release shown as a percentage of total cellular material w/ mean values ± SE for n = 5. *, P < 0.05; **, P < 0.01 for comparison w/ corresponding control samples. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29930202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-41385_goat-polyclonal-unc13d-munc-13-4-antibody-31020241612622.jpg "Western Blot: UNC13D/Munc 13-4 Antibody [NB100-41385] - Munc13-4 KD strongly impairs exosome release. (A) SDS-PAGE Western blot of indicated proteins in MDA-MB-231 cells after stable expression of shRNA for Munc13-4 or Rab27a or a scrambled control (Ctrl). (B) Culture medium from MDA-MB-231 cells either untreated or stimulated w/ 1.25 µM ionomycin for 30 min centrifuged at 1,000 g to remove cellular debris & 10,000 g to remove large extracellular vesicles. (C) The resulting 10,000-g supernatant filtered onto a nitrocellulose membrane & analyzed for CD63, CD9, ALIX, & GM130 content by antibody blotting. (D) Quantification of CD63, CD9, & ALIX blots in C are shown as exosome release as a percentage of total cellular material w/ mean values ± standard error (SE) for n ≥ 3. *, P < 0.05 for comparison w/ corresponding control samples. (E) Panc-1 or A549 cells left untreated or treated w/ TGF beta -1 for 24 h. Indicated proteins detected by SDS-PAGE Western blot. (F) Panc-1 cells left untreated or treated w/ TGF beta -1 for 24 h, & Munc13-4 levels determined by immunofluorescence. TGF beta -1–treated cells exhibited a mesenchymal morphology. Bars, 5 μm. (G) A549 cells stably expressing control shRNA (Ctrl) or Munc13-4 shRNA left untreated (Untr) or treated w/ TGF beta -1 for 24 h, & SDS-PAGE Western blotting for indicated proteins conducted. (H) Culture media supernatants (as in B) from A549 cells that either untreated or stimulated w/ 1.25 µM ionomycin for 30 min filtered onto nitrocellulose membrane & analyzed for CD63 & GM130. (I) Quantification of CD63+ exosome release shown as a percentage of total cellular material w/ mean values ± SE for n = 5. *, P < 0.05; **, P < 0.01 for comparison w/ corresponding control samples. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29930202), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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