Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.

Name: Human UCP2 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-82387
Price: 699 USD

Question

Accepted Answer

Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	ELISA
Suitable Sample Type	Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range	0.16 - 10 ng/mL
Sensitivity	0.09 ng/mL

Specificity	This kit recognizes Human UCP2 in samples. No Significant cross-reactivity or interference between Human UCP2 and analogues was observed.
Standard Curve Range	0.16 - 10 ng/mL
Sensitivity	0.09 ng/mL
Assay Type	Sandwich-ELISA
Inter-Assay	CV% < 5.36
Intra-Assay	CV% < 5.45
Spike Recovery	94-107%
Sample Volume	100 ul
Kit Type	ELISA Kit (Colorimetric)
Gene	UCP2

Human UCP2 ELISA Kit (Colorimetric)

Species Glossary

Images

Product Details

Order Details

Human UCP2 ELISA Kit (Colorimetric) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Kit Components

Alternate Names for Human UCP2 ELISA Kit (Colorimetric)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for UCP2 ELISA Kit (NBP2-82387) (0)

Reviews for UCP2 ELISA Kit (NBP2-82387) (0)

Product General Protocols

FAQs for UCP2 ELISA Kit (NBP2-82387). (Showing 1 - 1 of 1 FAQ).

Additional UCP2 Products

Blogs on UCP2

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Bioinformatics