![Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human gallbladder. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Two-pore-calcium-channel-protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30670-img0005.jpg "Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human gallbladder. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | A synthetic peptide from amino acid region 700-752 of human Two pore calcium channel protein 2 conjugated to blue carrier protein was used as the antigen. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TPCN2 |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin: Antigen retrieval is essential. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Sterilized water |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Ammonium sulfate precipitation |
| Reconstitution Instructions | Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format. |
![Bioactivity ASIP [Unconjugated]](https://images.novusbio.com/images2/ASIP_9094AG_1188.jpg)
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Two-pore-calcium-channel-protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30670-img0001.jpg "Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Two-pore-calcium-channel-protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30670-img0002.jpg "Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Two-pore-calcium-channel-protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30670-img0003.jpg "Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.")
![Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human lung. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.](http://images.novusbio.com/fullsize/Two-pore-calcium-channel-protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30670-img0004.jpg "Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human lung. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")