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Mucolipin 3 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Mucolipin 3 Antibody [NBP1-83791] - Staining in human adrenal gland and skeletal muscle tissues using anti-MCOLN3 antibody. Corresponding MCOLN3 RNA-seq data ...read more
Immunocytochemistry/ Immunofluorescence: Mucolipin 3 Antibody [NBP1-83791] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoli & cytosol.
Immunohistochemistry-Paraffin: Mucolipin 3 Antibody [NBP1-83791] - Staining of human adrenal gland shows high expression.
Immunohistochemistry-Paraffin: Mucolipin 3 Antibody [NBP1-83791] - Staining of human skeletal muscle shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Mucolipin 3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YENKGTKQSAMAICQHFYKRGNIYPGNDTFDIDPEIETECFFVEPDEPFHIGTPAENKLNLTLDFHRLLTVELQFKLKAINLQTVRHQELPDCYDFTLT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MCOLN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Mucolipin 3 Protein (NBP1-83791PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Mucolipin 3 Antibody

  • FLJ11006
  • FLJ36629
  • MCOLN3
  • MGC71509
  • Mucolipin 3
  • mucolipin-3
  • TRPML3
  • TRP-ML3

Background

SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in the cochlea; particularly in the inner and outer hair cells. DISEASE: Defects in Mcoln3 are the cause of the varitin-waddler (Va) phenotype. Classical Va mice exhibit early-onset hearing loss, vestibular defects, pigmentation abnormalities and perinatal lethality. The phenotype varitin-waddler Jackcon (Va-J), which arose in a cross segregating for Va, is similar but less severe.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MCOLN3