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TRPS1 Antibody [Unconjugated]

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Western blot shows lysates of MDA-MB-453 human breast cancer cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRPS1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TRPS1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of MDA-MB-453 human breast cancer cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRPS1 Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for TRPS1 at approximately 191 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4838-SP
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TRPS1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human TRPS1
Met1-Phe222
Accession # Q9UHF7
Specificity
Detects human TRPS1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRPS1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4838 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRPS1 Antibody [Unconjugated]

  • GC79
  • trichorhinophalangeal syndrome I
  • tricho-rhino-phalangeal syndrome type I protein
  • TRPS1

Background

TRPS1 (also GC79) is a 160-175 kDa (predicted 140 kDa) member of the zinc finger family of transcription factors. It is ubiquitously expressed and serves to repress the expression of GATA-regulated genes. Human TRPS1 is 1281 amino acids (aa) in length. It contains nine divergent zinc-finger motifs (aa 222‑1267), the seventh of which is associated with a GATA-binding sequence (aa 896‑920) and is flanked by two NLSs (aa 886‑891 and 946‑952). Two SUMOylation sites exist (Lys1192 and Lys1201) and when utilized, they each add 40 kDa to the native MW. One alternative splice form shows premature truncation after Lys853. A second shows an alternate start site 13 aa upstream of the standard start site. Over aa 1‑222, human TRPS1 shares 95% and 89% aa identity with canine and mouse TRPS1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRPS1 Antibody (AF4838)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ChIP, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(1)
Immunoprecipitation
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 5 of 5.
Publications using AF4838 Applications Species
J Zhang, X Lv, B Wei, X Gong, L Chen CHD4 mediates SOX2 transcription through TRPS1 in luminal breast cancer Cellular Signalling, 2022-09-06;100(0):110464. 2022-09-06 [PMID: 36075559] (Western Blot, Human) Western Blot Human
Y Wang, X Lin, X Gong, L Wu, J Zhang, W Liu, J Li, L Chen Atypical GATA transcription factor TRPS1 represses gene expression by recruiting CHD4/NuRD(MTA2) and suppresses cell migration and invasion by repressing TP63 expression Oncogenesis, 2018-12-19;7(12):96. 2018-12-19 [PMID: 30563971] (Immunoprecipitation, Western Blot, Human) Immunoprecipitation, Western Blot Human
X Gong, W Liu, L Wu, Z Ma, Y Wang, S Yu, J Zhang, H Xie, G Wei, F Ma, L Lu, L Chen Transcriptional repressor GATA binding 1-mediated repression of SRY-box 2 expression suppresses cancer stem cell functions and tumor initiation J. Biol. Chem., 2018-10-12;0(0):. 2018-10-12 [PMID: 30315105]
Y Wang, J Zhang, L Wu, W Liu, G Wei, X Gong, Y Liu, Z Ma, F Ma, JP Thiery, L Chen Tricho-rhino-phalangeal syndrome 1 protein functions as a scaffold required for ubiquitin-specific protease 4-directed histone deacetylase 2 de-ubiquitination and tumor growth Breast Cancer Res., 2018-08-02;20(1):83. 2018-08-02 [PMID: 30071870] (Western Blot, Human) Western Blot Human
AA Serandour, H Mohammed, A Miremadi, KW Mulder, JS Carroll TRPS1 regulates oestrogen receptor binding and histone acetylation at enhancers Oncogene, 2018-06-12;0(0):. 2018-06-12 [PMID: 29895970] (ChIP, Western Blot, Human) ChIP, Western Blot Human

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Bioinformatics

Gene Symbol TRPS1
Uniprot