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EIF3S3 Antibody

Images

 
Western Blot: EIF3S3 Antibody [NBP1-84870] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: EIF3S3 Antibody [NBP1-84870] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: EIF3S3 Antibody [NBP1-84870] - Staining of human duodenum shows strong cytoplasmic positivity in lymphoid cells.
Western Blot: EIF3S3 Antibody [NBP1-84870] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). ...read more
Immunohistochemistry-Paraffin: EIF3S3 Antibody [NBP1-84870] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells and cells in molecular layer.
Immunohistochemistry-Paraffin: EIF3S3 Antibody [NBP1-84870] - Staining of human cervix shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: EIF3S3 Antibody [NBP1-84870] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells and cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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EIF3S3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QQQQKHQYQQRRQQENMQRQSRGEPPLPEEDLSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGKLFMAQALQEYNN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF3H
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
EIF3S3 Protein (NBP1-84870PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EIF3S3 Antibody

  • eIF-3-gamma
  • eIF3-gamma
  • eIF3h
  • eIF3-p40
  • EIF3S3eIF3 p40 subunit
  • Eukaryotic translation initiation factor 3 subunit 3
  • eukaryotic translation initiation factor 3 subunit H
  • eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD)
  • eukaryotic translation initiation factor 3, subunit 3 (gamma, 40kD)
  • eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa
  • eukaryotic translation initiation factor 3, subunit H
  • MGC102958

Background

Eukaryotic initiation factor 3 subunit H (eIF3H) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3H is a non-conserved subunit and part of the functional core of eIF3. It may function to stabilize the eIF3 complex and stimulate protein synthesis. Many cancer cell lines have been shown to express high levels of eIF3H, and it may have an oncogenic role in colorectal cancer susceptibility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF3H