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TRF-1 Antibody

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Western Blot: TRF-1 Antibody [NB100-891] - Analysis of TRF1 in HeLa lysate (RIPA buffer, 30ug total protein per lane) using this antibody at 2 ug/ml. Primary incubated for 12 hour. Detected using chemiluminescence.

Product Details

Summary
Reactivity Hu, Po, BvSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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TRF-1 Antibody Summary

Immunogen
Peptide with sequence C-TMKKLKLISSDSED corresponding to C-Terminus according to NP_003209.2, NP_059523.2.
Marker
Telomere Marker
Specificity
This antibody is expected to recognise isoform 1 (NP_059523.2) and isoform 2 (NP_003209.2).
Predicted Species
Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TERF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 60-65 kDa band and 45 kDa band bands observed in HeLa lysate.
Control
HeLa Whole Cell Lysate
TRF-1 Overexpression Lysate
Publications
Read Publication using NB100-891.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRF-1 Antibody

  • NIMA-interacting protein 2
  • PIN-2
  • PIN2hTRF1-AS
  • Telomeric protein Pin2/TRF1
  • telomeric repeat binding factor (NIMA-interacting) 1
  • telomeric repeat binding factor 1
  • telomeric repeat binding protein 1
  • telomeric repeat-binding factor 1
  • TERF1
  • TRBF1
  • TRF1
  • TRF-1
  • TRF1FLJ41416
  • TRFt-TRF1
  • TTAGGG repeat-binding factor 1

Background

This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRF-1 Antibody (NB100-891)(1)


Showing Publication 1 - 1 of 1.
Publication using NB100-891 Applications Species
Chong L, van Steensel B, Broccoli D et al. A human telomeric protein. Science 1995-12-08 [PMID: 7502076]

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Bioinformatics

Gene Symbol TERF1
Entrez
Uniprot