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TRAIL/TNFSF10 Antibody [Unconjugated]

 (15 Publications)
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Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TRAIL/TNFSF10 at approximately 27 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) ...read more
TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) at 15 µg/mL overnight ...read more
TRAIL/TNFSF10 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human TRAIL/TNFSF10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF375) at 3 ...read more
In the presence of a cross-linking antibody, Mouse Anti-His Tag Monoclonal Antibody (Catalog # MAB050), and the metabolic inhibitor Actinomycin D, Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF375
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF375-SP
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TRAIL/TNFSF10 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human TRAIL/TNFSF10
Thr95-Gly281
Accession # P50591
Specificity
Detects human TRAIL/TNFSF10 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TRAIL is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFSF10
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization <140 ng/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF375 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAIL/TNFSF10 Antibody [Unconjugated]

  • Apo-2 ligand
  • APO2L
  • Apo-2Ltumor necrosis factor (ligand) family, member 10
  • APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
  • CD253 antigen
  • CD253
  • Protein TRAIL
  • TL2
  • TNF-related apoptosis-inducing ligand
  • TNFSF10
  • TRAIL
  • TRAILTNF-related apoptosis inducing ligand TRAIL
  • tumor necrosis factor (ligand) superfamily, member 10
  • tumor necrosis factor ligand superfamily member 10

Background

TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TRAIL/TNFSF10
Antibody [Unconjugate
AF375
TRAIL/TNFSF10 Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC, B/N

Publications for TRAIL/TNFSF10 Antibody (AF375)(15)

We have publications tested in 3 confirmed species: Human, Canine, Feline.

We have publications tested in 8 applications: Affinity Purification, Bioassay, Flow Cytometry, ICC, IHC, IHC-Fr, Neutralization, Western Blot.


Filter By Application
Affinity Purification
(1)
Bioassay
(1)
Flow Cytometry
(1)
ICC
(1)
IHC
(1)
IHC-Fr
(2)
Neutralization
(6)
Western Blot
(3)
All Applications
Filter By Species
Human
(14)
Canine
(1)
Feline
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF375 Applications Species
Yoon, Y;Kim, CW;Kim, MY;Baik, SK;Jung, PY;Eom, YW; Interferon-? Overexpression in Adipose Tissue-Derived Stem Cells Induces HepG2 and Macrophage Cell Death in Liver Tumor Organoids via Induction of TNF-Related Apoptosis-Inducing Ligand Expression International journal of molecular sciences 2024-01-22 [PMID: 38279326] (Western Blot, Human) Western Blot Human
Luo, Y;Hao, H;Wang, Z;Ong, C;Dutcher, R;Xu, Y;Liu, J;Pedersen, LC;Xu, D; Heparan sulfate promotes TRAIL-induced tumor cell apoptosis bioRxiv : the preprint server for biology 2023-07-26 [PMID: 37546770] (Flow Cytometry, Human) Flow Cytometry Human
Y An, J Jeon, L Sun, A Derakhshan, J Chen, S Carlson, H Cheng, C Silvin, X Yang, C Van Waes, Z Chen Death agonist antibody against TRAILR2/DR5/TNFRSF10B enhances birinapant anti-tumor activity in HPV-positive head and neck squamous cell carcinomas Scientific Reports, 2021-03-18;11(1):6392. 2021-03-18 [PMID: 33737574] (Neutralization, Human) Neutralization Human
LM Sanmarco, MA Wheeler, C Gutiérrez-, CM Polonio, M Linnerbaue, FA Pinho-Ribe, Z Li, F Giovannoni, KV Batterman, G Scalisi, SEJ Zandee, ES Heck, M Alsuwailm, DL Rosene, B Becher, IM Chiu, A Prat, FJ Quintana Gut-licensed IFN&amp;gamma+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes Nature, 2021-01-06;0(0):. 2021-01-06 [PMID: 33408417] (IHC, Human) IHC Human
Chun Sung Byun, Soonjae Hwang, Sung-Hun Woo, Moon Young Kim, Jin Suk Lee, Jong In Lee et al. Adipose Tissue-Derived Mesenchymal Stem Cells Suppress Growth of Huh7 Hepatocellular Carcinoma Cells via Interferon (IFN)-beta -Mediated JAK/STAT1 Pathway in vitro International Journal of Medical Sciences 2020-02-18 [PMID: 32210710]
T Li, Y Yang, H Song, H Li, A Cui, Y Liu, L Su, IN Crispe, Z Tu Activated NK cells kill hepatic stellate cells via p38/PI3K signaling in a TRAIL-involved degranulation manner J. Leukoc. Biol., 2019-02-12;0(0):. 2019-02-12 [PMID: 30748035] (Neutralization, Human) Neutralization Human
Mathieu J, Zhou W, Xing Y, Sperber H, Ferreccio A, Agoston Z, Kuppusamy K, Moon R, Ruohola-Baker H Hypoxia-inducible factors have distinct and stage-specific roles during reprogramming of human cells to pluripotency. Cell Stem Cell, 2014-03-20;14(5):592-605. 2014-03-20 [PMID: 24656769] (Neutralization, Human) Neutralization Human
Kim , Seong Mu, Woo , Ji Sun, Jeong , Chang Hy, Ryu , Chung He, Jang , Jae-Deog, Jeun , Sin-Soo Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma. Stem Cells Transl Med, 2014-01-16;3(2):172-82. 2014-01-16 [PMID: 24436439] (Western Blot, Human) Western Blot Human
Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N Human cytomegalovirus infection elicits new decidual natural killer cell effector functions. PLoS Pathog, 2013-04-04;9(4):e1003257. 2013-04-04 [PMID: 23592985] (Bioassay, Human) Bioassay Human
Navenot JM, Fujii N, Peiper SC KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis. Mol. Pharmacol., 2009-02-06;75(5):1074-83. 2009-02-06 [PMID: 19201817] (Neutralization, Human) Neutralization Human
Show All 15 Publications.

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Bioinformatics

Gene Symbol TNFSF10
Uniprot