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TNF RI/TNFRSF1A Antibody [Biotin]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF-425-PB
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF-425-PB
Carrier Free
AF-425-PB
Carrier Free
AF-425-SP
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TNF RI/TNFRSF1A Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant mouse TNF RI/TNFRSF1A
Ile22-Ala212
Accession # P25118
Specificity
Detects mouse TNF RI/TNFRSF1A in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant human (rh) TNF-alpha , recombinant rat TNF-alpha , recombinant porcine TNF-alpha , rhTNF-beta , recombinant mouse TNF sRII, rhTNF sRII, and rhTNF sRI is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFRSF1A
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Mouse TNF RI/TNFRSF1A Antibody (Catalog # MAB425)
ELISA Detection: Mouse TNF RI/TNFRSF1A Biotinylated Antibody (Catalog # BAF425)
Standard: Recombinant Mouse TNF RI/TNFRSF1A (Catalog # 425-R1)
Readout System
Publications
Read Publications using
BAF425 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF RI/TNFRSF1A Antibody [Biotin]

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

TNF receptor 1 (TNF RI; also called TNF R-p55/p60, TNFRSF1A and CD120a) is a type I transmembrane protein that belongs to the TNF receptor superfamily (1, 2). TNF RI is widely expressed and is present on the cell surface as a trimer of 55 kDa subunits. It serves as a receptor for both TNF‑ alpha and TNF‑ beta /lymphotoxin. Each subunit contains four TNF‑ alpha trimer-binding cysteine-rich domains (CRD) in its extracellular domain (ECD) (1‑6). TNF-alpha binding to TNF R1 induces the sequestration of TNFRI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (7, 8). Release of the 28‑34 kDa TNF RI ECD occurs constitutively, and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 7‑12). Full-length TNF RI may also be released in exosome-like vesicles (12). Such release helps to resolve inflammatory reactions as it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 13, 14). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (7, 15). Although there is a second receptor for TNF-alpha (TNF R2), TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). TNF R1 is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria monocytogenes (1‑3). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence and a 191 aa ECD with a PLAD domain (6). This mediates constitutive trimer formation. The PLAD domain is followed by four CRDs, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (16). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, procine, human and rat TNF RI, respectively; and it shows 23% aa identity with the ECD of TNF RII.

  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  4. Banner, D.W et al. (1993) Cell 73:431.
  5. Medvedev, A.E. et al. (1996) J. Biol. Chem. 271:9778.
  6. Chan, F.K. et al. (2000) Science 288:2351.
  7. Legler, D.F. et al. (2003) Immunity 18:655.
  8. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  9. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  10. Jin, L. et al. (2000) J. Immunol. 165:5153.
  11. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  12. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  13. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  14. McDermott, M.F. et al. (1999) Cell 97:133.
  15. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
  16. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TNF RI/TNFRSF1A Antibody (BAF425)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ELISA Development, IHC, Luminex Development, Western Blot.


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ELISA Development
(4)
IHC
(2)
Luminex Development
(1)
Western Blot
(1)
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Mouse
(8)
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Showing Publications 1 - 8 of 8.
Publications using BAF425 Applications Species
DG Kim, JW Choi, IJ Jo, MJ Kim, HS Lee, SH Hong, HJ Song, GS Bae, SJ Park Berberine ameliorates lipopolysaccharide?induced inflammatory responses in mouse inner medullary collecting duct?3 cells by downregulation of NF?kappaB pathway Mol Med Rep, 2019-11-19;0(0):. 2019-11-19 [PMID: 31746359] (ELISA Development, Mouse) ELISA Development Mouse
Wertz I, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman R, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother W, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee W, Goodnow C, Balazs M, Yu K, Kolumam G, Dixit V Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation. Nature, 2015-12-09;528(7582):370-5. 2015-12-09 [PMID: 26649818] (Western Blot, Mouse) Western Blot Mouse
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse) Luminex Development Mouse
Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis. J. Immunol., 2007-11-15;179(10):7166-75. 2007-11-15 [PMID: 17982109] (IHC, Mouse) IHC Mouse
Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance. Arthritis Rheum., 2007-03-01;56(3):949-59. 2007-03-01 [PMID: 17328072] (IHC, Mouse) IHC Mouse
Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006-08-01;177(3):1967-74. 2006-08-01 [PMID: 16849510] (ELISA Development, Mouse) ELISA Development Mouse
Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA Development, Mouse) ELISA Development Mouse
Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2005-02-26;231(1):103-11. 2005-02-26 [PMID: 15919375] (ELISA Development, Mouse) ELISA Development Mouse

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Bioinformatics

Gene Symbol TNFRSF1A
Uniprot