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TMC3 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: TMC3 Antibody [NBP1-86240] - Analysis in human parathyroid gland and liver tissues using NBP1-86240 antibody. Corresponding TMC3 RNA-seq data are presented ...read more
Immunocytochemistry/ Immunofluorescence: TMC3 Antibody [NBP1-86240] - Staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: TMC3 Antibody [NBP1-86240] - Staining of human tonsil shows no positivity as expected.
Immunohistochemistry-Paraffin: TMC3 Antibody [NBP1-86240] - Staining of human parathyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: TMC3 Antibody [NBP1-86240] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: TMC3 Antibody [NBP1-86240] - Staining of human duodenum shows no positivity in glandular cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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TMC3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RRNASQCYLYQESLLLSNLDDSFSADETGDSNDPEQIFQNIQFQKDLMANIRCRPWTMGQKLRALRQAKNIVLKFEGRLTRTRGYQAAG
Predicted Species
Mouse (92%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TMC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TMC3 Protein (NBP1-86240PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TMC3 Antibody

  • transmembrane channel-like 3
  • transmembrane channel-like protein 3

Background

SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Detected in most neuronal organs and also in some non-neuronal tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TMC3