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STT3A Antibody (4D4)

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Western Blot: STT3A Antibody (4D4) [H00003703-M02] - Analysis of ITM1 expression in transfected 293T cell line by ITM1 monoclonal antibody (M02), clone 4D4.Lane 1: ITM1 transfected lysate(80.5 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: STT3A Antibody (4D4) [H00003703-M02] - Analysis of monoclonal antibody to ITM1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
ELISA: STT3A Antibody (4D4) [H00003703-M02] - Detection limit for recombinant GST tagged ITM1 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clone
4D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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STT3A Antibody (4D4) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
ITM1 (NP_689926, 603 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GGSTDTGKHIKENDYYTPTGEFRVDREGSPVLLNCLMYKMCYYRFGQVYTEAKRPPGFDRVRNAEIGNKDFELDVLEEAYTTEHWLVRIYKVKDLDNRG
Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Specificity
ITM1 - integral membrane protein 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
STT3A
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactivity against recombinant protein for WB.
Publications
Read Publications using
H00003703-M02 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STT3A Antibody (4D4)

  • Integral membrane protein 1EC 2.4.1.119
  • integral transmembrane protein 1
  • ITM1dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
  • MGC9042
  • Oligosaccharyl transferase subunit STT3A
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S.cerevisiae)
  • STT3-AFLJ27038
  • TMCB5
  • transmembrane conserved
  • Transmembrane protein TMC

Background

Asparagine-linked glycosylation (ALG) is one of the most common protein modification reactions in eukaryotic cells, as many proteins that are translocated across or integrated into the rough endoplasmic reticulum (RER) carry N-linked oligosaccharides. STT3A is a subunit of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. SST3A seems to be involved in the complex substrate specificity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STT3A Antibody (H00003703-M02)(5)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol STT3A
Entrez
OMIM
Uniprot