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TIPIN Antibody

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Western Blot: TIPIN Antibody [NBP2-38860] - Analysis in control (vector only transfected HEK293T lysate) and TIPIN over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TIPIN Antibody [NBP2-38860] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & vesicles.
Orthogonal Strategies: Immunohistochemistry-Paraffin: TIPIN Antibody [NBP2-38860] - Staining in human testis and prostate tissues using anti-TIPIN antibody. Corresponding TIPIN RNA-seq data are presented for the ...read more
Immunohistochemistry: TIPIN Antibody [NBP2-38860] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: TIPIN Antibody [NBP2-38860] - Staining of human prostate shows low expression as expected.
Immunohistochemistry-Paraffin: TIPIN Antibody [NBP2-38860] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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TIPIN Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: HEAEDLKMLIRHMEHWAHRLFPKLQFEDFIDRVEYLGSKKEVQTCLKRIRLDLPILHEDFVSNNDEVAENNEHDVTSTELDPFLTNLSESEMFASELS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TIPIN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TIPIN Protein (NBP2-38860PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TIPIN Antibody

  • FLJ20516
  • TIMELESS interacting protein
  • TIMELESS-interacting protein

Background

Timeless-interacting protein (TIPIN) was originally isolated as a protein that interacts with timeless, a factor that plays a role in circadian rhythm and cell cycle checkpoint regulation. The TIM-TIPIN complex has been shown to coordinate the intra-S-phase checkpoint in response to UV-induced DNA damage. TIPIN is required for cell cycle arrest in response to DNA damage. It also interacts with RPA (replication protein A) on DNA and is able to slow down DNA chain elongation in response to UV-damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TIPIN
Uniprot