Human peripheral blood mono-nuclear cells (PBMCs) were stained with Rat Anti-Human TIM-3 Alexa Fluor® 700-conjugated Mono-clonal Antibody (Catalog # FAB2365N) and Mouse Anti-Human CD14 PE-conjugated Mono-clonal ...read more
Upregulation of immune checkpoint ligands on T cells after dendritic cell stimulation. T cells of 7–14 healthy donor were cocultured with autologous TLR-3-DCs pulsed with CMV, EBV, influenza, tetanus (CEFT) peptide ...read more
Clone 344823 was used by HLDA to establish CD designation
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIM‑3 Ser22-Arg200 Accession # Q8TDQ0.2
Specificity
Detects human TIM‑3 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) TIM‑1, rhTIM‑4, recombinant mouse (rm) TIM‑1, rmTIM‑2, rmTIM‑3, rmTIM‑5, or rmTIM‑6.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
HAVCR2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for TIM-3 Antibody (344823) [Alexa Fluor® 700]
CD366
FLJ14428
HAVCR2
HAVcr-2
hepatitis A virus cellular receptor 2
kidney injury molecule-3
KIM-3
SPTCL
T cell immunoglobulin mucin 3
T cell immunoglobulin mucin-3
T-cell immunoglobulin and mucin domain-containing protein 3
TIM 3
TIM3 T-cell membrane protein 3
TIM3
TIM-3
TIMD3
TIMD-3
TIMD3KIM-3
Background
TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1-3). There are three TIM genes in human and eight in mouse. Mature human TIM-3 consists of a 181 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail (4). An alternately spliced isoform is truncated following a short substitution after the Ig-like domain. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is expressed on the surface of effector T cells (CD4+ Th1 and CD8+ Tc1) but not on helper T cells (CD4+ Th2 and CD8+ Tc2) (4, 5). In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types. It also occurs on hippocampal neurons (7-10). The Ig domain of TIM-3 interacts with a ligand on resting but not activated Th1 and Th2 cells (5, 11). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9. This induces TIM-3 Tyr phosphorylation and proapoptotic signaling (8, 12). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation and cytotoxicity (5, 10, 11, 13), regulatory T cell development (5), and increases in macrophage and neutrophil infiltration into sites of inflammation (14). Soluble mouse TIM-3 constructs which lack the cytoplasmic domain have been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (5, 15).
Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
Monney, L. et al. (2002) Nature 415:536.
Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
Khademi, M. et al. (2004) J. Immunol. 172:7169.
Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
Oikawa, T. et al. (2006) J. Immunol. 177:4281.
Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.
Geng, H. et al. (2006) J. Immunol. 176:1411.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
TIM-3, a critical immune checkpoint in HIV research CD4+ T-helper cells (Th) are the white blood lymphocytes expressing surface glycoprotein antigen CD4. These T-helper cells play an important role in the adaptive immune system by releasing T cell cytokines that help other immune cells to suppress o... Read full blog post.
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