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Galectin-9 Antibody [Unconjugated]

Images

 
Western blot shows conditioned media from HT-29 human colon adenocarcinoma cell line, human peripheral blood lymphocytes (PBL), and human dendritic cells untreated (-) or treated (+) with 10 µg/mL PHA for 6 days. PVDF ...read more
Galectin-9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Human Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
Galectin‑9 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human Galectin‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2045) at 0.3 µg/mL for 1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Galectin-9 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Galectin-9
Specificity
Detects human Galectin-9 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LGALS9
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.3-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF2045 in the following applications:

Publications
Read Publications using
AF2045 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-9 Antibody [Unconjugated]

  • Ecalectin
  • GAL9
  • gal-9
  • galectin 9
  • Galectin9
  • Galectin-9
  • HUAT
  • lectin, galactoside-binding, soluble, 9
  • LGALS9
  • LGALS9A
  • MGC117375
  • MGC125973
  • MGC125974
  • Tumor antigen HOM-HD-21
  • urate transporter/channel protein

Background

Galectin-9 (also known as Ecalectin and UAT) is a 40 kDa beta -galactosidase binding lectin that belongs to a tandem repeat group of Galectins. Activated cells such as T cells, PMNs, astrocytes, fibroblasts and endothelial cells can express Galectin-9, but its release requires select MMPs. Human to mouse, there is 69% aa sequence identity for Galectin-9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-9 Antibody (AF2045)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 9 applications: Binding Assay, Cell Culture, Flow Cytometry, ICC, IHC-Fr, IHC-P, Immunohistochemistry, Neutralization, Western Blot.


Filter By Application
Binding Assay
(1)
Cell Culture
(1)
Flow Cytometry
(3)
ICC
(1)
IHC-Fr
(2)
IHC-P
(7)
Immunohistochemistry
(1)
Neutralization
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(14)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using AF2045 Applications Species
Ling, J;You, S;Chen, W;Yang, X;Xv, Y;Zhu, B; Galectin-9 as a new biomarker of acute-on-chronic liver failure Scientific reports 2024-09-27 [PMID: 39333198] (Immunohistochemistry, Human) Immunohistochemistry Human
Santalla M�ndez R, Rodgers Furones A, Classens R et al. Galectin-9 interacts with Vamp-3 to regulate cytokine secretion in dendritic cells Cell Mol Life Sci 9/27/2023 [PMID: 37755527]
Santalla M�ndez R, Rodgers Furones A, Classens R et al. Galectin-9 interacts with Vamp-3 to regulate cytokine secretion in dendritic cells Cell Mol Life Sci 2023-09-27 [PMID: 37755527]
MA Nielsen, D Køster, AY Mehta, K Stengaard-, P Busson, P Junker, K Hørslev-Pe, ML Hetland, M Østergaard, M Hvid, H Leffler, TW Kragstrup, RD Cummings, B Deleuran Increased Galectin-9 Levels Correlate with Disease Activity in Patients with DMARD-Na�ve Rheumatoid Arthritis and Modulate the Secretion of MCP-1 and IL-6 from Synovial Fibroblasts Cells, 2023-01-15;12(2):. 2023-01-15 [PMID: 36672263] (Binding Assay, Human) Binding Assay Human
Stephanie J. Si, Gerald B. Wertheim, David M. Barrett High Expression of TIM 3 and Galectin 9 on Immunohistochemistry Staining of Tumor Specimen at Diagnosis in Pediatric Patients with Ewing Sarcoma J Cancer Immunol (Wilmington) 6/30/2022 [PMID: 35938052]
Marco Frensch, Christina Jäger, Peter F. Müller, Annamaria Tadić, Isabel Wilhelm, Sarah Wehrum et al. Bacterial lectin BambL acts as a B cell superantigen Cellular and Molecular Life Sciences 12/1/2021 [PMID: 34731252]
Qin Chen, Xiaoming Zhou, Ruonan Hou, Zhiliang Zhou, Zhiyi Wang, Ying Chen et al. Aminophylline modulates the permeability of endothelial cells via the Slit2‑Robo4 pathway in lipopolysaccharide‑induced inflammation Experimental and Therapeutic Medicine 7/21/2021 [PMID: 34373728]
JN Sudhakar, HH Lu, HY Chiang, CS Suen, MJ Hwang, SY Wu, CN Shen, YM Chang, FA Li, FT Liu, JW Shui Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas Nat Commun, 2020-08-27;11(1):4286. 2020-08-27 [PMID: 32855403] (Western Blot, Mouse) Western Blot Mouse
K Sideras, RA de Man, SM Harrington, WG Polak, G Zhou, HM Schutz, A Pedroza-Go, K Biermann, S Mancham, BE Hansen, R Bart Takke, AJ van Vuuren, Q Pan, JNM Ijzermans, S Sleijfer, D Sprengers, H Dong, J Kwekkeboom, MJ Bruno Circulating levels of PD-L1 and Galectin-9 are associated with patient survival in surgically treated Hepatocellular Carcinoma independent of their intra-tumoral expression levels Sci Rep, 2019-07-23;9(1):10677. 2019-07-23 [PMID: 31337865] (IHC-P, Human) IHC-P Human
X Zhang, D He, S Gao, Y Wei, L Wang Aspergillus�fumigatus enhances human NK cell activity by regulating M1 macrophage polarization Mol Med Rep, 2019-06-06;20(2):1241-1249. 2019-06-06 [PMID: 31173233] (Cell Culture, Western Blot, Human) Cell Culture, Western Blot Human
Show All 22 Publications.

Review for Galectin-9 Antibody (AF2045) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF2045:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Galectin-9 AF2045
Enlarge
5
reviewed by:
Verified Customer
IHC-P Human 04/04/2020
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBreast cancer tissue
SpeciesHuman
LotKNI0117011

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Recent Reviews

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Verified Customer
04/04/2020
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol LGALS9