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Galectin-9 Antibody [Biotin]

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Galectin‑9 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Galectin‑9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2045) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Galectin‑9 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Galectin‑9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2045) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF2045
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF2045
Carrier Free
AF2045
Carrier Free
AF2045-SP
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Galectin-9 Antibody [Biotin] Summary

Immunogen
E. coli-derived recombinant human Galectin-9
Specificity
Detects human Galectin-9 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Galectin-3 and rhGalectin-4 is observed and less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-7, and rhGalectin-8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LGALS9
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Readout System
Publications
Read Publications using
BAF2045 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-9 Antibody [Biotin]

  • Ecalectin
  • GAL9
  • gal-9
  • galectin 9
  • Galectin9
  • Galectin-9
  • HUAT
  • lectin, galactoside-binding, soluble, 9
  • LGALS9
  • LGALS9A
  • MGC117375
  • MGC125973
  • MGC125974
  • Tumor antigen HOM-HD-21
  • urate transporter/channel protein

Background

Galectin-9 (also known as Ecalectin and UAT) is a 40 kDa beta -galactoside binding lectin that belongs to a tandem repeat group of Galectins. Activated cells such as T cells, PMNs, astrocytes, fibroblasts and endothelial cells can express Galectin-9, but its release requires select MMPs. Human to mouse, there is 69% aa sequence identity for Galectin-9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-9 Antibody (BAF2045)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ELISA Development (Detection), Flow Cytometry, Western Blot.


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(1)
Flow Cytometry
(1)
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(1)
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(2)
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Bioinformatics

Gene Symbol LGALS9