Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant ...read more
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tpo Ser22-Gly353 Accession # P40225
Specificity
Detects human Tpo in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Tpo and recombinant rat Tpo is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
THPO
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Thrombopoietin/THPO Antibody [Unconjugated]
Megakaryocyte colony-stimulating factor
Megakaryocyte growth and development factor
megakaryocyte stimulating factor
MGDF
MGDFC-mpl ligand
MKCSF
MK-CSF
ML
MPL ligand
MPLLG
MPLLGMGC163194
Myeloproliferative leukemia virus oncogene ligand
THCYT1
THPO
thrombopoietin nirs variant 1
Thrombopoietin
Tpo
TPOMKCSF
Background
Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1-3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10, 13). It is cleaved by platelet-derived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). TheC-terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (15, 16).
Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
Li, J. et al. (1999) Br. J. Haematol. 106:345.
Bartley, T.D. et al. (1994) Cell 77:1117.
de Sauvage, F.J. et al. (1994) Nature 369:533.
Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
Kaushansky, K. et al. (1994) Nature 369:568.
Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
Broudy, V.C. et al. (1995), Blood 85:1719.
Lok, S.I. et al. (1994) Nature 369:565.
Chen, J. et al. (1995) Blood 86:4054.
Oda, A. et al. (1996) Blood 87:4664.
Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
Samoylenko, A. et al. (2008) Cell. Signal. 20:154.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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