TAF15 Antibody Summary
Immunogen |
Recombinant protein encompassing a sequence within the center region of human TAF15. The exact sequence is proprietary. |
Localization |
Nucleus |
Predicted Species |
Porcine (91%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
TAF15 |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:100-1:1000
- Immunohistochemistry 1:100-1:1000
- Immunohistochemistry-Paraffin 1:100-1:1000
- Immunoprecipitation 1:10 - 1:500
- Western Blot 1:500-1:3000
|
Theoretical MW |
62 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Negative Xenopus reactivity reported by customer review.
Packaging, Storage & Formulations
Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
Buffer |
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
Preservative |
0.01% Thimerosal |
Purity |
Antigen Affinity-purified |
Alternate Names for TAF15 Antibody
Background
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for TAF15 Antibody (NBP1-32461) (0)
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Product General Protocols
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Video Protocols
FAQs for TAF15 Antibody (NBP1-32461). (Showing 1 - 1 of 1 FAQ).
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Has anyone ever tried this antibody in Xenopus? If so, what is the reference?
- This antibody has not yet been tested in Xenopus. There are only unreviewed Xenopus TAF15 sequences on UniProt, so I am not sure how accurate they are. I carried out a sequence comparison, and the percent homology with the human immunogen looks to be about 40-50%, which is quite low, suggesting that the antibody probably won't bind, unfortunately. If you did wish to try this antibody with Xenopus, note that you would be eligible for our Innovator's Rewards program, where Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we would send you a form for you to submit a summary of your research and report your experimental results, after which your award would be given, regardless of whether or not the antibody proved suitable for the species.
Secondary Antibodies
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