![Western Blot: STAG2 Antibody [NB100-301] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody : Affinity purified rabbit anti-SA2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/STAG2-Antibody-Western-Blot-NB100-301-img0007.jpg "Western Blot: STAG2 Antibody [NB100-301] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody : Affinity purified rabbit anti-SA2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A 31 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 2 encoded in part by exon 31 and 32 (LocusLink ID 10735). |
| Specificity | NB100-301 is specific for human SA2 protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | STAG2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-301 | Applications | Species |
|---|---|---|
| Zhang N, Pati D. C-terminus of Sororin interacts with SA2 and regulates sister chromatid cohesion Cell Cycle. 2015-01-21 [PMID: 25608232] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | STAG2 |
![Immunocytochemistry/Immunofluorescence: STAG2 Antibody [NB100-301] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.](http://images.novusbio.com/fullsize/STAG2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-301-img0002.jpg "Immunocytochemistry/Immunofluorescence: STAG2 Antibody [NB100-301] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.")
![Immunoprecipitation: STAG2 Antibody [NB100-301] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SA2 antibody used for IP at 6 ug per reaction. SA2 was also immunoprecipitated by rabbit anti-SA2 antibodies. For blotting immunoprecipitated SA2,was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/STAG2-Antibody-Immunoprecipitation-NB100-301-img0010.jpg "Immunoprecipitation: STAG2 Antibody [NB100-301] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SA2 antibody used for IP at 6 ug per reaction. SA2 was also immunoprecipitated by rabbit anti-SA2 antibodies. For blotting immunoprecipitated SA2,was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
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