![Western Blot: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SF1.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-OTI7H9-Azide-and-BSA-Free-Western-Blot-NBP2-74338-img0001.jpg "Western Blot: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SF1.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | OTI7H9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-276 of human SF1 (NP_973727) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SF1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended. |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SF1 |
![Immunohistochemistry: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - Staining of paraffin-embedded Human lymphoma tissue using anti-SF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-OTI7H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74338-img0005.jpg "Immunohistochemistry: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - Staining of paraffin-embedded Human lymphoma tissue using anti-SF1 mouse monoclonal antibody.")
![Immunohistochemistry: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-OTI7H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74338-img0002.jpg "Immunohistochemistry: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SF1 mouse monoclonal antibody.")
![Immunohistochemistry: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-OTI7H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74338-img0003.jpg "Immunohistochemistry: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SF1 mouse monoclonal antibody.")
![Immunohistochemistry: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - Staining of paraffin-embedded Human bladder tissue within the normal limits using anti-SF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-OTI7H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74338-img0004.jpg "Immunohistochemistry: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - Staining of paraffin-embedded Human bladder tissue within the normal limits using anti-SF1 mouse monoclonal antibody.")
![Immunohistochemistry: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SF1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Splicing-Factor-1-Antibody-OTI7H9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74338-img0006.jpg "Immunohistochemistry: Splicing Factor 1 Antibody (OTI7H9) - Azide and BSA Free [NBP2-74338] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SF1 mouse monoclonal antibody.")
![Immunocytochemistry SOX9 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX9_AF3075_Immunocytochemistry_15995.jpg)
![Western Blot SOX9 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX9_AF3075_Western_Blot_21580.jpg)
![Immunocytochemistry SOX9 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX9_AF3075_Immunocytochemistry_6490.jpg)
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")