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SOX9 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining in human testis and skeletal muscle tissues . Corresponding SOX9 RNA-seq data are presented for the same tissues.
Genetic Strategies: Knockdown Validated: SOX9 Antibody [NBP1-85551] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-SOX9 antibody. Remaining relative ...read more
Immunocytochemistry/ Immunofluorescence: SOX9 Antibody [NBP1-85551] - Further analysis of P2X7-EGFP expressing cells in the dentate gyrus and CA1 region. Co-staining of EGFP with the alternative astrocyte marker SOX9 in ...read more
Western Blot: SOX9 Antibody [NBP1-85551] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: SOX9 Antibody [NBP1-85551] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining of human colorectal cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.
Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining of human small intestine shows moderate nuclear positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: SOX9 Antibody [NBP1-85551] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.
Simple Western: SOX9 Antibody [NBP1-85551] - Simple Western lane view shows a specific band for SOX9 in 0.1 mg/ml of U-251MG sp (left) and HepG2 (right) lysate. This experiment was performed under reducing conditions ...read more
Simple Western: SOX9 Antibody [NBP1-85551] - Electropherogram image(s) of corresponding Simple Western lane view. SOX9 antibody was used at 1:100 dilution on U-251MG sp and HepG2 lysates(s).

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, GS, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SOX9 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SQRTHIKTEQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTR
Marker
Sertoli Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SOX9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Gel Supershift Assay Reported in scientific literature (PMID: 26430891).
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Frozen Reported in scientific literature (PMID:32103177).
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Knockdown Validated
  • Simple Western
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100br/>In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control Peptide
SOX9 Protein (NBP1-85551PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-85551 in the following applications:

Publications
Read Publications using
NBP1-85551 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:33645550). Porcine reactivity reported in scientific Iliterature (PMID: 26430891). Use in Canine reported in scientific literature (PMID:26428883).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SOX9 Antibody

  • campomelic dysplasia, autosomal sex-reversal
  • CMD 1
  • CMD1
  • CMPD1
  • SOX9
  • SRA1SRY (sex-determining region Y)-box 9 protein
  • SRY (sex determining region Y)-box 9
  • SRY-related HMG-box, gene 9
  • transcription factor SOX-9

Background

SOX9 is a member of the family of SOX (Sry-type highmobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, IHC, GS, KD

Publications for SOX9 Antibody (NBP1-85551)(21)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Porcine.

We have publications tested in 7 applications: EMSA, IF/IHC, IHC, IHC-Fr, IHC-P, IHF-Fr, WB.


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EMSA
(1)
IF/IHC
(7)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
IHF-Fr
(1)
WB
(4)
All Applications
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Human
(4)
Mouse
(5)
Rat
(5)
Canine
(1)
Porcine
(1)
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using NBP1-85551 Applications Species
Waters BJ, Birman ZR, Wagner MR et al. Islet architecture in adult mice is actively maintained by Robo2 expression in ? cells Developmental biology 2023-11-15 [PMID: 37972678] (IHC, Mouse) IHC Mouse
Lu JH, Chueh KS, Juan TJ et al. Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat International journal of molecular sciences 2023-05-04 [PMID: 37175945] (WB, Rat) WB Rat
Deepe R, Drummond J, Wolters R et al. Sox9 Expression in the Second Heart Field; A Morphological Assessment of the Importance to Cardiac Development with Emphasis on Atrioventricular Septation Journal of Cardiovascular Development and Disease 2022-11-02 [PMID: 36354775] (IHC-Fr, Mouse) IHC-Fr Mouse
Baek I, Bello AB, Jeon J et al. Therapeutic potential of epiphyseal growth plate cells for bone regeneration in an osteoporosis model Journal of tissue engineering 2022-08-11 [PMID: 35983547] (WB, Rat)

Details:
EGPCs and BM-MSCs were isolated from 8 weeks old Sprague Dawley (SD) male rat. Dilution used 1:1000.
WB Rat
Liu Q, Guo Q, Guo W et al. Loss of CEP70 function affects acrosome biogenesis and flagella formation during spermiogenesis Cell death & disease 2021-05-12 [PMID: 33980814] (IF/IHC, Mouse) IF/IHC Mouse
Winkler A, Wrzos C, Haberl M et al. Blood-brain barrier resealing in neuromyelitis optica occurs independently of astrocyte regeneration The Journal of clinical investigation 2021-03-01 [PMID: 33645550] (Rat) Rat
Deepe R, Fitzgerald E, Wolters R et al. The Mesenchymal Cap of the Atrial Septum and Atrial and Atrioventricular Septation J Cardiovasc Dev Dis 2020-11-04 [PMID: 33158164] (IF/IHC, Mouse) IF/IHC Mouse
van Gastel N, Stegen S, Eelen G et al. Lipid availability determines fate of skeletal progenitor cells via SOX9 Nature 2020-02-26 [PMID: 32103177] (IHF-Fr, IHC-P, Mouse) IHF-Fr, IHC-P Mouse
Tournaire G, Stegen S, Giacomini G et al. Nestin-GFP transgene labels skeletal progenitors in the periosteum Bone 2020-04-01 [PMID: 32036051]
Cheng B, Liu Y, Zhao Y et al. The role of anthrax toxin protein receptor 1 as a new mechanosensor molecule and its mechanotransduction in BMSCs under hydrostatic pressure Sci Rep 2019-09-02 [PMID: 31477767] (WB, Rat) WB Rat
Show All 21 Publications.

Review for SOX9 Antibody (NBP1-85551) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-85551:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SOX9 NBP1-85551
Enlarge
5
reviewed by:
Verified Customer
WB Human 01/30/2019
View

Summary

ApplicationWestern Blot
Sample Tested293T lysates
SpeciesHuman
LotF115596

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOX9 Antibody (NBP1-85551) (0)

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Secondary Antibodies

 

Isotype Controls

Additional SOX9 Products

Research Areas for SOX9 Antibody (NBP1-85551)

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Blogs on SOX9.

Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients
By Michalina Hanzel, PhDAlexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an ...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.

SOX9 - Be careful, I can reverse your gender!
SOX9 is a member of the SOX family of HMG DNA-binding domain transcription factors. The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. cAMP and protein kinase A (PKA) st...  Read full blog post.

Using the Hif-1 Alpha Antibody in Prostate Cancer Research
The Hypoxia-inducible Factor 1 (HIF1) protein is a heterodimeric transcription factor which plays an important role in mammalian oxygen homeostasis in conditions of hypoxia, or low oxygen concentration. HIF-1 alpha antibody reagents are widely used in...  Read full blog post.

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Recent Reviews

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Verified Customer
01/30/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol SOX9
Entrez
Uniprot