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SOX17 Antibody [Allophycocyanin]

 (15 Publications)
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Definitive endoderm-differentiated (Catalog # SC019) BG01V human embryonic stem cells were stained with Goat Anti-Human SOX17 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1924A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Definitive endoderm-differentiated (Catalog # SC019) BG01V human embryonic stem cells were stained with Goat Anti-Human SOX17 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1924A, filled ...read more
Addition of DMSO to Activin A/low serum-based medium increases the efficiency of definitive endoderm differentiation.(A) Flow cytometry analysis of hESC and DE cells double-stained for the pluripotency marker OCT4 and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF1924
NL557
NL1924R
Unconjugated
AF1924
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF1924
Carrier Free
AF1924
Carrier Free
NL1924R
Carrier Free
AF1924-SP
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SOX17 Antibody [Allophycocyanin] Summary

Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Specificity
Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX6, and rhSOX15 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX17
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC1924A in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX17 Antibody [Allophycocyanin]

  • FLJ22252
  • SOX17
  • SRY (sex determining region Y)-box 17
  • SRY-related HMG-box transcription factor SOX17
  • transcription factor SOX-17
  • VUR3

Background

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2). In Western blot, AF1924 detects a 60 kDa band of endogenous SOX17 which is higher than predicted molecular weight but consistent with the current literature (3).

 

  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
  3. Sohn, J. et al. (2006) J. Neuroscience 26:9722.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX17 Antibody (IC1924A)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, ICC, IHC.


Filter By Application
Flow Cytometry
(6)
ICC
(1)
IHC
(1)
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Human
(7)
Mouse
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using IC1924A Applications Species
Juhyung Jung, Yun Chang, Gyuhyung Jin, Qing Deng, Xiaojun Lance Lian, Xiaoping Bao Chemically defined generation of human definitive hematopoietic stem and progenitor cells STAR Protocols 2023-03-01 [PMID: 36527716]
Yun Chang, Ramizah Syahirah, Xuepeng Wang, Gyuhyung Jin, Sandra Torregrosa-Allen, Bennett D. Elzey et al. Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy Cell Reports 2022-07-19 [PMID: 35858579]
Seri Choi, Jiwoong Choi, Seongmin Cheon, Jihong Song, Seung-Yeon Kim, Ji-eun Kim et al. Pulmonary fibrosis model using micro-CT analyzable human PSC–derived alveolar organoids containing alveolar macrophage-like cells Cell Biology and Toxicology 2022-08-01 [PMID: 35267148]
Grace A. Christopher, Rebecca J. Noort, Jessica L. Esseltine Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification Biomolecules 2021-12-22 [PMID: 35053163]
J Liu, T Pan, Y Chen, Y Liu, F Yang, Q Chen, N Abbas, M Zhong, Q Zhang, Y Xu, YX Li Repair of acute liver damage with immune evasive hESC derived hepato-blasts Stem Cell Res, 2020-09-26;49(0):102010. 2020-09-26 [PMID: 33011360] (ICC, IHC, Human, Mouse) ICC, IHC Human, Mouse
Mahaddalkar PU, Scheibner K, Pfluger S et al. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm Nature Biotechnology 2020-09-01 [PMID: 32341565]
N Bredenkamp, J Yang, J Clarke, GG Stirparo, F von Meyenn, S Dietmann, D Baker, R Drummond, Y Ren, D Li, C Wu, M Rostovskay, S Eminli-Mei, A Smith, G Guo Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency Stem Cell Reports, 2019-11-07;13(6):1083-1098. 11/7/2019 [PMID: 31708477]
D Sheyn, S Ben-David, W Tawackoli, Z Zhou, K Salehi, M Bez, S De Mel, V Chan, J Roth, P Avalos, JC Giaconi, H Yameen, L Hazanov, D Seliktar, D Li, D Gazit, Z Gazit Human iPSCs can be differentiated into notochordal cells that reduce intervertebral disc degeneration in a porcine model Theranostics, 2019-10-12;9(25):7506-7524. 2019-10-12 [PMID: 31695783] (Flow Cytometry, Human) Flow Cytometry Human
J Lu, A Baccei, E Lummertz d, C Guillermie, S McManus, LA Finney, C Zhang, ML Steinhause, H Li, PH Lerou Single-cell RNA sequencing reveals metallothionein heterogeneity during hESC differentiation to definitive endoderm Stem Cell Res, 2018-01-31;28(0):48-55. 2018-01-31 [PMID: 29427839] (Flow Cytometry, Human) Flow Cytometry Human
Yijie Geng, Bradley Feng Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis Heliyon 2015-12-05 [PMID: 26705539]
Show All 15 Publications.

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Bioinformatics

Gene Symbol SOX17
Uniprot