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SOD3/EC-SOD Antibody [Unconjugated]

 (10 Publications)
 (1 Review)
 
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Western blot shows lysates of mouse lung and kidney tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Mouse SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant mouse SOD1, SOD2, and SOD3 (5 ng/lane) were included. A specific band for SOD3/EC-SOD was detected at approxi-mately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of mouse lung and kidney tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Mouse SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4817) followed by HRP-conjugated ...read more
Simple Western shows lysates of mouse aorta, loaded at 0.5 mg/ml. A specific band was detected for SOD3/EC‑SOD at approximately 40 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse SOD3/EC‑SOD Antigen ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF4817-SP
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SOD3/EC-SOD Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse SOD3/EC‑SOD
Ser25-Thr251
Accession # O09164
Specificity
Detects mouse SOD3/EC‑SOD in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse SOD1 or SOD2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOD3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF4817 in the following applications:
Publications
Read Publications using
AF4817 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOD3/EC-SOD Antibody [Unconjugated]

  • ECSOD
  • EC-SOD
  • EC-SODEC 1.15.1.1
  • extracellular superoxide dismutase [Cu-Zn]
  • MGC20077
  • SOD3
  • superoxide dismutase 3, extracellular

Background

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 3, SOD3, also known as extracellular (EC) SOD, is tetrameric glycoprotein with an apparent subunit molecular weight of about 30 kDa. Like SOD1, SOD3 binds one Cu2+ and Zn2+ ions per subunit but differs in sequence and tissue distribution. Three isoenzymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD3 shares 23% and 17% sequence identity with SOD1 and SOD2, respectively. Mouse SOD3 shares ~66% and 82% sequence homology with human and rat SOD3, respectively. SOD3 is a secretory protein and is synthesized with a putative 24-amino acid signal peptide preceding the 227 amino acids in the mature SOD3. SOD3 is found in plasma, lymph, and synovial fluid as well as in tissues. SOD3 binds on the surface of endothelial cells through the heparan sulfate proteoglycan and eliminates the oxygen radicals from the NADP-dependent oxidative system of neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOD3/EC-SOD Antibody (AF4817)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(5)
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(5)
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Showing Publications 1 - 10 of 10.
Publications using AF4817 Applications Species
H Fujiyoshi, T Egawa, E Kurogi, T Yokokawa, K Kido, T Hayashi TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle International Journal of Molecular Sciences, 2022-02-07;23(3):. 2022-02-07 [PMID: 35163799] (Western Blot, Mouse) Western Blot Mouse
Lee DE, Brown JL, Rosa-Caldwell ME et al. Cancer-induced Cardiac Atrophy Adversely Affects Myocardial Redox State and Mitochondrial Oxidative Characteristics JCSM Rapid Communications 3/11/2021 [PMID: 33693448]
Lee DE, Brown JL, Rosa-Caldwell ME et al. Cancer-induced Cardiac Atrophy Adversely Affects Myocardial Redox State and Mitochondrial Oxidative Characteristics JCSM Rapid Communications 2021-03-11 [PMID: 33693448]
Megan E. Rosa-Caldwell, Conner A. Benson, David E. Lee, Jacob L. Brown, Tyrone A. Washington, Nicholas P. Greene et al. Mitochondrial Function and Protein Turnover in the Diaphragm are Altered in LLC Tumor Model of Cancer Cachexia International Journal of Molecular Sciences 10/22/2020 [PMID: 33105841]
S Lim, KR Dunlap, ME Rosa-Caldw, WS Haynie, LT Jansen, TA Washington, NP Greene Comparative plasma proteomics in muscle atrophy during cancer-cachexia and disuse: The search for atrokines Physiol Rep, 2020-10-01;8(19):e14608. 2020-10-01 [PMID: 33063952] (Western Blot, Mouse) Western Blot Mouse
M Hohl, M Mayr, L Lang, AG Nickel, J Barallobre, X Yin, T Speer, SR Selejan, C Goettsch, K Erb, C Fecher-Tro, JC Reil, B Linz, S Ruf, T Hübschle, C Maack, M Böhm, T Sadowski, D Linz Cathepsin A contributes to left ventricular remodeling by degrading extracellular superoxide dismutase in mice J. Biol. Chem., 2020-07-09;0(0):. 2020-07-09 [PMID: 32647007] (Western Blot, Mouse) Western Blot Mouse
H Zhao, BM Lewellen, RJ Wilson, D Cui, JC Drake, M Zhang, Z Yan Long-term voluntary running prevents the onset of symptomatic Friedreich's ataxia in mice Sci Rep, 2020-04-08;10(1):6095. 2020-04-08 [PMID: 32269244] (Western Blot, Mouse) Western Blot Mouse
Brown JL, Rosa-Caldwell ME, Lee DE et al. Mitochondrial degeneration precedes the development of muscle atrophy in progression of cancer cachexia in tumour-bearing mice J Cachexia Sarcopenia Muscle. 12/1/2017 [PMID: 28845591]
Roderick J. Tan, Dong Zhou, Liangxiang Xiao, Lili Zhou, Yingjian Li, Sheldon I. Bastacky et al. Extracellular Superoxide Dismutase Protects against Proteinuric Kidney Disease Journal of the American Society of Nephrology 10/1/2015 [PMID: 25644107]
Riad A, Bien S, Westermann D, Becher PM, Loya K, Landmesser U, Kroemer HK, Schultheiss HP, Tschope C Pretreatment with statin attenuates the cardiotoxicity of Doxorubicin in mice. Cancer Res., 2009-01-15;69(2):695-9. 2009-01-15 [PMID: 19147586] (Western Blot, Mouse) Western Blot Mouse

Review for SOD3/EC-SOD Antibody (AF4817) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF4817:
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WB
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  5
reviewed by:
Verified Customer
 WB Mouse 01/08/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23886589
SpeciesMouse

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 Verified Customer
01/08/2015
Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol SOD3
Uniprot