Home » SMPD3/NSMase2 » SMPD3 Antibodies » SMPD3/NSMase2 Antibody (758612) [Unconjugated]

SMPD3/NSMase2 Antibody (758612) [Unconjugated]

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Western blot shows lysates of CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMPD3 Monoclonal Antibody (Catalog # MAB7184) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SMPD3 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMPD3 Monoclonal Antibody (Catalog # MAB7184) followed by HRP-conjugated Anti-Mouse IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
758612
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB7184-SP
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SMPD3/NSMase2 Antibody (758612) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human SMPD3
Val2-Ala655
Accession # Q9NY59
Specificity
Detects human SMPD3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) SMPD1 or rhPP2A is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SMPD3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB7184 in the following application:
Publications
Read Publication using MAB7184.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SMPD3/NSMase2 Antibody (758612) [Unconjugated]

  • EC 3.1.4.12
  • FLJ22593
  • MGC138443
  • Neutral sphingomyelinase 2
  • Neutral sphingomyelinase II
  • NSMASE2
  • nSMase-2
  • SMPD3
  • sphingomyelin phosphodiesterase 3
  • sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinaseII)

Background

SMPD3 (Sphingomyelin phosphodiesterase 3; also Neutral sphingomyelinase 2/nSMase2) is a 69-74 kDa member of the neutral sphingomyelinase family of enzymes. It is a monomeric Golgi/plasma membrane enzyme that converts sphingomyelin (a plasma membrane lipid) into ceramide and phosphorylcholine. This generates second messenger components that participate in signal transduction. Human SMPD3 is a two transmembrane, 655 amino acids molecule. It contains an N-terminal luminal segment (aa 1-10), a cytoplasmic region (aa 32-64), and one catalytic domain (aa 340-646). Phosphorylation increases its MW to 78 kDa in SDS-PAGE. There is one potential isoform that possesses an Asn substitution for aa 569-587. Over aa 2-655, human SMPD3 shares 91% aa sequence identity with mouse SMPD3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SMPD3/NSMase2
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MAB7184
SMPD3/NSMase2 Antibody (758612) [Unconjugated]
Species: Hu
Applications: WB

Publications for SMPD3/NSMase2 Antibody (MAB7184)(1)


Review for SMPD3/NSMase2 Antibody (MAB7184) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB7184:
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(1)
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(1)
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Western Blot SMPD3/NSMase2 MAB7184
Enlarge 		4
reviewed by:
Sebastian Silvera
 WB Mouse 08/22/2017
View

Summary

ApplicationWestern Blot
Sample TestedSkeletal muscle tissue
SpeciesMouse
LotCGKN0112021

Comments

CommentsSMPD3 in white Gastrocnemius muscle of mouse at protein concentrations of 2.5, 5, 10 and 20ug (Left to right). On PVDF membrane.

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FAQs for SMPD3/NSMase2 Antibody (MAB7184) (0)

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Secondary Antibodies

 

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Recent Reviews

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Western Blot SMPD3/NSMase2 MAB7184
Enlarge
 Sebastian Silvera
08/22/2017
Application: WB
Species: Mouse
read this review

Bioinformatics

Gene Symbol SMPD3
Uniprot