Novus Biologicals products are now on bio-techne.com

Smoothened Antibody (1D9)

Images

 
Western Blot: Smoothened Antibody (1D9) [H00006608-M11] - Analysis of SMO expression in A-431 (Cat # L015V1).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
1D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Smoothened Antibody (1D9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
SMO (NP_005622.1, 653 a.a. ~ 787 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NLWLVEAEISPELQKRLGRKKKRRKRKKEVCPLAPPPELHPPAPAPSTIPRLPQLPRQKCLVAAGAWGAGDSCRQGAWTLVSNPFCPEPSPPQDPFLPSAPAPVAWAHGRRQGLGPIHSRTNLMDTELMDADSDF
Specificity
SMO (1D9)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SMO
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Smoothened Antibody (1D9)

  • bnb
  • CRJS
  • FZD11
  • Gx
  • Protein Gx
  • SMO
  • SMOH
  • SMOHseven transmembrane helix receptor
  • smoothened (Drosophila) homolog
  • smoothened homolog (Drosophila)
  • smoothened homolog
  • Smoothened

Background

Smoothened, a Frizzled Receptor, is homologous to the Drosophila segment polarity Smo gene. It associates with the Patched protein to mediate the cellular response to the Hedgehog secreted protein signal, which controls patterning and growth during vertebrate development. Smoothened is activated in a number of human tumors and can function as an oncogene in basal-cell carcinomas. Smoothened expression has been documented in bladder, blood, brain, the central nervous system, skin, and ureter. ESTs have been isolated from amnion, bone, brain, breast, embryo, eye, heart, nerve, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00002523-P01
Species: Hu
Applications: ELISA, AP, PA, WB
H00003077-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
MAB41051
Species: Hu, Mu
Applications: IHC, WB
NB600-600
Species: Ca, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, IB, ICC/IF, IHC, IHC-P, KD, WB
AF482
Species: Mu
Applications: IHC, WB
NBP1-33581
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P
NBP3-41370
Species: Hu, Po
Applications: ICC/IF, IHC, WB
NBP1-85351
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF1705
Species: Mu
Applications: IHC, WB
AF3635
Species: Mu
Applications: IHC, WB
NBP3-04509
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
AF3690
Species: Hu, Mu
Applications: ChIP, ICC, WB
NBP1-87384
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF1568
Species: Mu
Applications: WB
NBP1-83411
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-92172
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF196
Species: Mu
Applications: IHC, WB

Publications for Smoothened Antibody (H00006608-M11) (0)

There are no publications for Smoothened Antibody (H00006608-M11).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Smoothened Antibody (H00006608-M11) (0)

There are no reviews for Smoothened Antibody (H00006608-M11). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Smoothened Antibody (H00006608-M11) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Smoothened Products

Research Areas for Smoothened Antibody (H00006608-M11)

Find related products by research area.

Blogs on Smoothened.

The role of Smoothened in pulmonary pathologies
The Hedgehog (Hh) family of secreted proteins is involved in a number of developmental processes, one of which is the development of cancer. Past data suggests that the Sonic hedgehog (Shh) receptor is composed of two transmembrane proteins, Patche...  Read full blog post.

Pancreatic Cancer Research Targets Hedgehog Signaling Pathway
The Hedgehog Signaling Pathway (HSP) is an important pathway involved in embryonic development by regulating cell differentiation. This pathway has also become an increasingly hot topic in cancer research in recent years. The HSP involves the interact...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Smoothened Antibody (1D9) and receive a gift card or discount.

Bioinformatics

Gene Symbol SMO
Entrez
OMIM
Uniprot