SMC1 Antibody (BL-205-2G8) Summary
Additional Information |
Recombinant Monoclonal Antibody. |
Immunogen |
Immunogen sequence between amino acids 1200 and 1233 (C-Term) |
Isotype |
IgG |
Clonality |
Monoclonal |
Host |
Rabbit |
Gene |
SMC1A |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
- Immunoprecipitation 20 ul/mg lysate
- Western Blot 1:1000
|
Packaging, Storage & Formulations
Storage |
Store at 2 - 8 C / 1 year from date of receipt |
Buffer |
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA |
Preservative |
0.09% Sodium Azide |
Purity |
Antigen Affinity-purified |
Notes
This Product sold under License from Bethyl Laboratories, Inc.
Alternate Names for SMC1 Antibody (BL-205-2G8)
Background
Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for SMC1 Antibody (NBP2-76412) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for SMC1 Antibody (NBP2-76412). (Showing 1 - 2 of 2 FAQ).
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I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?
- My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.
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My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.
- We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.
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