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Slug Antibody (OTI2B11)

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Western Blot: Slug Antibody (OTI2B11) [NBP2-45980] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Slug.
Western Blot: Slug Antibody (OTI2B11) [NBP2-45980] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Slug.
Immunohistochemistry: Slug Antibody (OTI2B11) [NBP2-45980] - Analysis of Human pancreas tissue.
Flow Cytometry: Slug Antibody (OTI2B11) [NBP2-45980] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by SNAI2 antibody.
Immunohistochemistry: Slug Antibody (OTI2B11) [NBP2-45980] - Analysis of Human tonsil tissue.
Immunofluorescence: Slug Antibody (OTI2B11) [NBP2-45980] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Slug.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74235AF350
Alexa Fluor 405
NBP2-74235AF405
Alexa Fluor 488
NBP2-74235AF488
Alexa Fluor 532
NBP2-74235AF532
Alexa Fluor 594
NBP2-74235AF594
Alexa Fluor 647
NBP2-74235AF647
Alexa Fluor 700
NBP2-74235AF700
Alexa Fluor 750
NBP2-74235AF750
Allophycocyanin
NBP2-74235APC
Allophycocyanin/Cy7
NBP2-74235APCCY7
Biotin
NBP2-74235B
CoraFluor 1
NBP2-74235CL1
DyLight 350
NBP2-74235UV
DyLight 405
NBP2-74235V
DyLight 488
NBP2-74235G
DyLight 550
NBP2-74235R
DyLight 594
NBP2-74235DL594
DyLight 650
NBP2-74235C
DyLight 680
NBP2-74235FR
DyLight 755
NBP2-74235IR
FITC
NBP2-74235F
HRP
NBP2-74235H
Janelia Fluor 525
NBP2-74235JF525
Janelia Fluor 549
NBP2-74235JF549
Janelia Fluor 585
NBP2-74235JF585
Janelia Fluor 635
NBP2-74235JF635
Janelia Fluor 646
NBP2-74235JF646
Janelia Fluor 669
NBP2-74235JF669
PE
NBP2-74235PE
PE/Atto594
NBP2-74235PEATT594
PE/Cy5.5
NBP2-74235PECY55
PE/Cy7
NBP2-74235PECY7
PerCP
NBP2-74235PCP
mFluor Violet 450 SE
NBP2-74235MFV450
mFluor Violet 500 SE
NBP2-74235MFV500
mFluor Violet 610 SE
NBP2-74235MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74235
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Slug Antibody (OTI2B11) Summary

Immunogen
Full length human recombinant protein of human SNAI2 (NP_003059) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SNAI2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Slug Antibody (OTI2B11)

  • MGC10182
  • Neural crest transcription factor Slug
  • Protein snail homolog 2
  • slug homolog, zinc finger protein (chicken)
  • Slug
  • SLUGH
  • SLUGH1
  • SLUGzinc finger protein
  • SNAI2
  • snail 2
  • snail homolog 2 (Drosophila)
  • SNAIL2
  • WS2D
  • zinc finger protein SNAI2

Background

SLUG encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
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Bioinformatics

Gene Symbol SNAI2