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Slug Antibody (4B6D5) - BSA Free

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Western Blot: Slug Antibody (4B6D5) [NBP2-52570] - Analysis using Slug mouse mAb against MCF-7 cell lysate.
Western Blot: Slug Antibody (4B6D5) [NBP2-52570] - Analysis using Slug mouse mAb against MCF-7 cell lysate.
Immunocytochemistry/ Immunofluorescence: Slug Antibody (4B6D5) [NBP2-52570] - Analysis of Hela cells using SNAI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry-Paraffin: Slug Antibody (4B6D5) [NBP2-52570] - Analysis of colon cancer tissues using SNAI2 mouse mAb with DAB staining.
Flow Cytometry: Slug Antibody (4B6D5) [NBP2-52570] - Analysis of MCF-7 cells using SNAI2 mouse mAb (green) and negative control (red).
Western Blot: Slug Antibody (4B6D5) [NBP2-52570] - Analysis using SNAI2 mAb against HEK293 (1) and SNAI2 (AA: 100-200)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Slug Antibody (4B6D5) [NBP2-52570] - Analysis using SNAI2 mAb against human SNAI2 (AA: 100-200) recombinant protein. (Expected MW is 39.8 kDa)
Immunohistochemistry-Paraffin: Slug Antibody (4B6D5) [NBP2-52570] - Analysis of cervical cancer tissues using SNAI2 mouse mAb with DAB staining.
ELISA: Slug Antibody (4B6D5) [NBP2-52570] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
4B6D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-52570-0.025mg
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Slug Antibody (4B6D5) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Slug (AA: 100-200) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SNAI2
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Slug Antibody (4B6D5) - BSA Free

  • MGC10182
  • Neural crest transcription factor Slug
  • Protein snail homolog 2
  • slug homolog, zinc finger protein (chicken)
  • Slug
  • SLUGH
  • SLUGH1
  • SLUGzinc finger protein
  • SNAI2
  • snail 2
  • snail homolog 2 (Drosophila)
  • SNAIL2
  • WS2D
  • zinc finger protein SNAI2

Background

SLUG encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol SNAI2